A sensitive rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) method was developed for exact and sensitive enumeration of subdominant bacterial populations. Using group- or species-specific primers for 16S or 23S rRNA, analytical curves were constructed for Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Clostridium perfringens, and Pseudomonas aeruginosa, and the t...

Bacterial populations inhabiting ultrapure water (UPW) systems were investigated. The analyzed UPW systems included pilot scale, bench scale, and full size UPW plants employed in the semiconductor and other industries. Bacteria present in the polishing loop of the UPW systems were enumerated by both plate counts and epifluorescence microscopy. Assessment of bacterial presence in UPW by epifluor...

erodium (geranaiceae) species are distributed in different habitats of iran. some species are of medicinal importance while some are well known weeds. there are no chromosome counts for erdoium species of iran. chromosome numbers are given for six taxa of erodium (geraniaceae) from iran: e. cicutarium (2n=36), e. ciconium(2n=18), e. malacoides (2n=20), e. moschatum (2n=20), e. neuradifolium (2n...

abstract: the growth rate of two probiotic strains with documented health effects was studied in ultra-high temperature treated milk separately and beside each other. the probiotic strains were lactobacillus acidophilus (la-5) and bifidobacterium lactis (bb-12). fermentation was followed for 12 h at 37 ˚c and the samples were analyzed for ph, titratable acidity and viable counts of probiotic ba...

Fish-borne illness is associated with pathogenic bacteria, Vibrio spp., transmitted from contaminated aquaculture water to fish. But little is known about the correlation between water quality in aquaculture and subsequent bacterial contamination in fish products. The degree of bacterial transmission from Vibrio spp. contaminated aquaculture water to fish was investigated. V. anguillarum, V. pa...

BACKGROUND Next-generation 16S ribosomal RNA gene sequencing is widely used to determine the relative composition of the mammalian gut microbiomes. However, in the absence of a reference, this does not reveal alterations in absolute abundance of specific operational taxonomic units if microbial loads vary across specimens. RESULTS Here we suggest the spiking of exogenous bacteria into crude s...

Background and Objective: This study was undertaken to assess the ability of standard urinalysis (UA) and hemocytometer white blood cell (WBC) counts for the diagnosis of urinary tract infection (UTI) in patients with urinary symptoms. Materials and Methods: A total of 600 patients with symptoms of urina...

the effects of essential oils (eos) of oregano and nutmeg with and without commercial emulsifier/stabilizer compound (e/s) on the microbial quality of ready-to-cook barbecued chicken were evaluated. barbecued chicken was traditionally prepared. 3 μl/g and 10 mg/g of eos and/or e/s were then added to the barbecued chicken, respectively. the samples were stored at 3°c for 144 h, 8 and 20ºc for 72...

this study was conducted to compare the effect of various commercial in-feed disinfectants on layer performance, intestinal microflora, and egg cholesterol. one hundred and eight layer hens (lsl, 24 wks of age) were divided into six groups consisting: control, formaycin-200 ppm, formalin-200 ppm, and nanosilver in feed-15 ppm or drinking water-7.5 ppm. there were no differences between experime...

Cuturable heterotrophic bacterial counts (viable counts: VC) by surface plating on ZoBell medium and total bacterial counts (TC) by epifluorescence microscopy were carried out in sea water off the southwest coast of lndia during the monsoon. TC were three orders of magnitude higher than VC. Pseudomonas/Alteromonas group 1 was dominant in both nearshore and offshore regions. Pigmented bacteria w...