In general, four different expression systems, namely, bacterial, yeast, baculovirus, and mammalian, are widely used for the overproduction of biochemical enzymes and therapeutic proteins. Clearly, bacterial expression systems offer ease of maneuverability with respect to large-scale production of recombinant proteins, while, a baculovirus expression system ensures proper protein modifications,...

Baculovirus F (fusion) proteins are found in the envelopes of budded virions. Recently a Drosophila melanogaster gene (CG4715) that encodes a protein with sequence similarity to baculovirus F proteins was discovered. To examine similarities and differences with baculovirus F proteins, we cloned the D. melanogaster cellular F (Dm-cF) protein gene and analyzed Dm-cF expression and localization. T...

The baculovirus expression vector system (BEVS) is widely established as a highly useful and effective eukaryotic expression system. Thousands of soluble and membrane proteins that, in general, are correctly folded, modified, sorted and assembled to produce highly authentic recombinant proteins have been cloned and expressed. This historical chronology and perspective will focus on the original...

This paper describes the expression of a cloned African horsesickness virus (AHSV) serotype 5 VP2-gene by a baculovirus recombinant that was generated by the BAC-TO-BAC system. Immunization of horses with crude cell lysates containing recombinant baculovirus-expressed AHSV5 VP2 did induce neutralizing antibodies, but afforded only partial protection against virulent virus challenge. Further ana...

Protein Expression of the Human Norovirus Capsid Gene using the Baculovirus Expression System Ji-Young Jin1, Chul Jong Park2, Seung-Won Park and Soon-Young Paik* Department of Microbiology, College of Medicine, The Catholic University, Seoul; Department of Dermatology, College of Medicine, The Catholic University, Seoul; Department of Agricultural Biology, National Academy of Agricultural Scien...

background: herpes simplex virus type 2 (hsv-2) is highly prevalent and major cause of genital herpes in humans. the life-long nature of infection and the increasing prevalence of genital herpes imply that vaccination is the best strategy for controlling the spread of infection and limiting hsv disease. hsv glycoprotein d (gd) is one of the most important viral immunogen which has an essential ...