نتایج جستجو برای: banding
تعداد نتایج: 7221 فیلتر نتایج به سال:
Homogeneous polymer solutions are well known to exhibit viscoelastic flow instabilities: purely elastic when inertia is negligible and inertio-elastic otherwise. Recently, shear-banding wormlike micelle solutions were also discovered to follow a similar phenomenology. In the shear-banding regime, inertia is usually negligible so only purely elastic flows have been reported. Here, we investigate...
The distribution of accessible antigenic sites in the chromosomal protein high mobility group one (HMG-1) in Chironomus thummi polytene chromosomes is visualized by immunofluorescence. The results indicate that (a) HMG-1 is distributed in a distinct banding pattern along the entire length of the chromosomes; (b) the banding pattern obtained with fluorescent antibody does not strictly correspond...
A comparative chromosome banding analysis of Chironomussolitus Linevich & Erbaeva, 1971 and Chironomusanthracinus Zetterstedt, 1860 from East Siberia (Lakes Baikal, Gusinoe, Arakhley and Irkutsk Reservoir) showed close similarity of banding sequences. Chironomussolitus differs from Chironomusanthracinus in one species-specific sequence of arm B. Arms C (43%) and D (30%) had inversion banding se...
C-banding techniques detect the presence of constitutive heterochromatin, which is usually located in centromeric regions of chromosomes in the majority of analysed species. The common method for C-banding used over the last 30 years involves treatment with a mild alkali barium hydroxide 5% Ba(OH)2 at 50 degrees C for 5-15 min and subsequent incubation in salt solution (2 x SSC at 60 degrees C ...
Ten germplasms of three Crotalaria species viz. C. pallida (Acc. No. 4250, 4803, 4805, 4806, and 4807), incana 4790, 4801, 4804, 4809), juncea (Local) were investigated cytogenetically through Chromomycin A3 (CMA) 4′-6 diamidino-2-phenylindole (DAPI) fluorescent bandings. After CMA-banding, 10 showed 31 centromeric 34 terminal bands which indicated a tendency accumulating GC-rich sequences at r...
background: ct scan and nuclear medicine exams deliver a great part of medical exposures. this study examined professional radiation hazards in ct scan and nuclear medicine workers. methods : in a cross sectional study 30 occupationally exposed workers and 7 controls (all from personnel of a laboratory) were selected. physical dosimetry was performed for exposed workers. blood samples were ob...
The technique of pulse-field gel electrophoresis (PFGE) makes use of restriction enzymes that cleave DNA at specific restriction sites, generating DNA fragments of different sizes. These fragments can be separated according to size by agarose-gel electrophoresis and visualized using DNA stains (e.g. ethidium bromide), giving a characteristic banding pattern. Given the high specificity of restri...
BACKGROUND The recent article by Guller, Klein, Hagen was reviewed and discussed by the authors of this response to critically analyze the validity of the conclusions, at a time when patients and providers depend on peer reviewed data to guide their health care choices. The authors of this response all have high volume bariatric surgery practices encompassing experience with both gastric bypass...
Nearly all methods of chromosome banding rely on harvesting chromosomes in mitosis. This is usually achieved by treating cells with tubulin inhibitors, such as colchicine or demecolcine (colcemid), that depolymerize the mitotic spindle and so arrest the cell at this stage. Excessively long incubations with Colcemid result in overcondensed chromosomes that band poorly and moreover some cell type...
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