Address for correspondence: Dr Eleonora Nucera, Allergy Unit, Università Cattolica del Sacro Cuore, Policlinico “A. Gemelli”, Largo F. Vito, 1-00168 Rome, Italy, phone: +39 0630154965, fax: +39 0630156999, e-mail: [email protected] Received: 10.02.2016, accepted: 9.07.2016. Anaphylaxis by antihistamine containing bovine gelatin: the utility of the basophil activation test in the dia...

Abbreviations: BAT: Basophil Activation Test; CRTH2: G proteincoupled Receptor-44 or Chemoattractant Receptor Expressed on Th2 cells; EDTA-K3: Ethylene-Diamine-Tetracetic Acid; ENPP-3: Ectoenzyme ectonucleotide Pyrophosphatase/Phosphodiesterases 3; IL: Interleukin; ISM: Indolent Systemic Mastocytosis; LAMP: Lysosomal Membrane Proteins; MFI: Median Fluorescence Intensity; PGD2: Prostaglandin D2;...

In recent years, the quantification of basophil activation by flow cytometry (basophil activation test, BAT) has proven to be a useful tool for the assessment of immediate-type responses to allergens mediated by IgE or other mechanisms in drug-allergic patients. To date, most BAT studies reported in the literature have used CD69 or CD203c as markers to quantify basophil activation after antigen...

Basophil activation was observed in patients with a history of carboplatin-induced severe hypersensitivity reaction (HR). However, the precise mechanism by which carboplatin induces basophil activation and the associated surrogate markers remains to be elucidated. To investigate whether IgE-dependent mechanisms, including the overexpression of FcεRI, participate in carboplatin-induced basophil ...

Background The basophil activation test (BAT) is an in vitro test which allows to identify children with food allergies at the sensitization stage and clinical manifestations of atopic dermatitis\\eczema (AD). The aim of our study was to observe the BAT in children with food allergies, optimize the diagnosis compared with other tests, and select an elimination diet. Early detection of sensitiza...