نتایج جستجو برای: bead array

تعداد نتایج: 140843  

2007
Pan Du Warren A. Kibbe Simon M. Lin Robert H. Lurie

Gene Identifier v.s. Oligonucleotide Identifier Oligonucleotides (often simply referred to as oligos or oligomers), which are typically between 25 to 75 bases long, have been extensively used by DNA microarray manufacturers to detect the expression of different genes. An oligonucleotide sequence that is located on a specific bead or position on a microarray is usually referred to as the probe s...

Journal: :Analytical biochemistry 2009
Hongna Liu Song Li Lishang Liu Lan Tian Nongyue He

To fulfill the increasing need for large-scale genetic research, we have developed a new solid-phase single base extension (SBE) protocol on magnetic nanoparticles (MNPs) for multiplex SNP detection using adapter polymerase chain reaction (PCR) products as templates. Extension primers were covalently immobilized on the MNPs, and allele-specific extension took place along the stretch of target D...

Journal: :Memorias do Instituto Oswaldo Cruz 2009
Caroline de Souza Almeida Clarice Abramo Caio César de Souza Alves Luciano Mazzoccoli Ana Paula Ferreira Henrique Couto Teixeira

Chemokines recruit and activate leukocytes, assisting granuloma formation. Herein, we evaluated plasma chemokines in patients with active tuberculosis (ATB) and after completing treatment (TTB) and compared them to BCG-vaccinated healthy controls (HC). Levels of chemokines were measured by cytometric bead array. Levels of CXCL8, CXCL9 and CXCL10 were higher in ATB patients compared to HC, but t...

2012
Daan Witters Karel Knez Kris Janssen Bert Verbruggen Robert Puers Jeroen Lammertyn

We present a novel strategy for patterning single magnetic beads by using digital microfluidics (DMF). A droplet containing a suspension of superparamagnetic particles is transported back and forth over an array of femtoliter-sized microwells by using electrowetting-on-dielectric actuation forces. Compared to existing methods, this technique allows patterning of superparamagnetic beads in micro...

2005
Komkrit Chomsuwan Sotoshi Yamada Takeshi Hagino Teerasak Somsak Masayoshi Iwahara

This paper describes the recognition of micro non-magnetic (conductive) and ferromagnetic material with spinvalve magneto-resistance (SV-GMR) sensor. High frequency and static magnetic fields were applied to material for the recognition of the micro conductive and ferromagnetic material respectively. The SV-GMR sensor is used to detect the magnetic field variation occurred wherever the material...

Journal: :Journal of Neuroimmunology 2013
Stephen Correia Ronald Cohen Assawin Gongvatana Skye Ross James Olchowski Kathryn Devlin Karen Tashima Bradford Navia Suzanne Delamonte

Chronic systemic immune activation and inflammatory processes have been linked to brain dysfunction in medically stable HIV-infected people. We investigated the association between verbal memory performance and plasma concentrations of 13 cytokines measured using multiplexed bead array immunoassay in 74 HIV-seropositive individuals and 50 HIV-seronegative controls. Memory performance was positi...

2006
Lars Toellner Martin Fischlechner Boris Ferko Reingard Maria Grabherr Edwin Donath

Background: Suspension array technology has surpassed ELISA for automated, simultaneous detection and quantification of soluble biomarkers such as virusspecific antibodies. We describe assays in which antigens are attached to a lipid bilayer surrounding colorcoded particles. Methods: We used layer-by-layer technology to establish a multiplex suspension array with distinguishable microbeads coat...

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