To findnovel cytoplasmic binding partners of the 1D-adrenergic receptor (AR), a yeast two-hybrid screen using the 1D-ARC terminus as bait was performed on a human brain cDNA library. -Syntrophin, a protein containing one PDZ domain and two pleckstrin homology domains, was isolated in this screen as an 1D-AR-interacting protein. -Syntrophin specifically recognized theC terminus of 1Dbut not 1Aor...

The OV20.0 virulence factor of orf virus antagonizes host antiviral responses. One mechanism through which it functions is by inhibiting activation the dsRNA-activated protein kinase R (PKR) sequestering dsRNA and physically interacting with PKR. Sequence alignment indicated that several key residues critical for binding were conserved in OV20.0, their contribution to OV20.O function was invest...

The G protein-coupled receptor (GPCR) µ-opioid (µOR) is one of several drug targets commercially available pain therapeutics. Various opioid drugs, such as morphines, have been associated with numerous substance abuse-related deaths around the world. Targeting allosteric sites can preclude this undesirable side effect. In addition, understanding underlying mechanism ligands in µOR highly sought...

BackgroundThe clinical outcome of hypertrophic cardiomyopathy patients is not only determined by the disease-causing mutation but influenced a variety disease modifiers. Here, we defined role location and mutant protein dose troponin T mutations I79N, R94C R278C.Methods resultsWe myofilament function after exchange in permeabilized single human cardiomyocytes as well cardiac patient samples har...

Abstract We identified Rho Related BTB Domain Containing 1 (RhoBTB1) as a key regulator of phosphodiesterase 5 (PDE5) activity, and through PDE5, vascular tone. the binding interface for PDE5 on RhoBTB1 by truncating full-length into its component domains. Co-immunoprecipitation analyses revealed that C-terminal half containing two domains domain (B1B2C) is minimal region required recruitment s...

Using site-directed mutagenesis, we have undertaken a study of a potential IGF-binding site in the C-terminal domain of rat IGFBP-5, lying close to or within a previously described heparin-binding domain (residues 201-218) in this protein. After analysis of binding activity using three different methods - ligand blotting, solution phase equilibrium binding and biosensor measurement of real-time...

Calmodulin (CaM) directly interacts with the aquaporin 0 (AQP0) C-terminus in a calcium dependent manner to regulate the water permeability of AQP0. We previously identified a missense mutation (p.R233K) in the putative CaM binding domain of AQP0 C-terminus in a congenital cataract family. This study was aimed at exploring the potential pathogenesis of this mutation causative of cataract and ma...

The TATA binding protein (TBP) and transcription factor IIB (TFIIB) play crucial roles in transcription of class II genes. The requirement for TBP-TFIIB interactions was evaluated in maize cells by introducing mutations into the Arabidopsis TBP (AtTBP2) within the C-terminal stirrup. Protein binding experiments indicated that amino acid residues E-144 and E-146 of AtTBP2 are both essential for ...