Trypsin has been encapsulated in the mesopores of a hierarchical mesoporous silica material synthesized via Cu(I) catalyzed azide-alkyne click reaction between azide functionalized large spherical SBA-15 particles and alkyne functionalized mesoporous silica nanoparticles (MSNs). Encapsulated trypsin functions as an efficient biocatalyst and can be recycled several times.

Herein, we synthesized 2,3-diaminophenazine (DAP) from o-phenylenediamine (OP) using fungal laccase as a biocatalyst. The conversion ratio of OP monomer was 85% and the yield of the final purified product, DAP, was 63%. The structure of the main product, DAP, was confirmed by using several spectroscopy techniques (UV-VIS, IR, 2DNMR, and MS).

Bacillus pumilus is a Gram-positive, rod-shaped, aerobic bacterium isolated from the soil. B. pumilus strain B6033 was originally selected as a biocatalyst for the stereospecific oxidation of β-lactams. Here, we present a 3.8-Mb assembly of its genome, which is the second fully assembled genome of a B. pumilus strain.

Highly selective enzymatic reductions of aldehydes to the corresponding alcohols was performed using an E. coli JM109 whole cell biocatalyst. A selective enzymatic method for the reduction of aldehydes could provide an eco-compatible alternative to chemical methods. The simplicity, fairly wide scope and the very high observed chemoselectivity of this approach are its most unique features.

Dynamic kinetic resolution (DKR) is one of the most attractive methods for enantioselective synthesis. In reported studies, lipase B from Candida antarctica (CALB) immobilized on siliceous mesoporous cellular foams (MCF) functionalized with different hydrophobic groups, and two ruthenium complexes substituted cyclopentadienyl ligands were investigated as catalysts chemoenzymatic DKR (rac)-1-phe...

A novel fusion protein has been rationally designed, combining the hexameric glutamate dehydrogenase from Clostridium symbiosum with dimeric formate Candida boidinii . The former enzyme consumes ammonia for reductive amination of α-ketoglutarate using NADH, while latter biocatalyst regenerates continuously cofactor. This enzymes opens new perspectives detection and removal ammonia. bifunctional...

The use of biocatalysis to create chiral centres for chemical pharmaceuticals is rapidly becoming a standard industrial technology. Often, the compounds of interest are poorly soluble in aqueous solvents leading to the use of organic co­ solvents to deliver substrate into the environment of the biocatalyst. Unfortunately these co-solvents can impact negatively on the stability of the biocatalys...