The Front Cover shows the bioconjugation of an antiproliferative prochelator to serum albumin via formation a disulfide bond with reduced cysteine on protein. abundance in blood is represented by red background featuring cells. disulfide-masked prochelators this class have activities at submicromolar levels panel cancer cell lines. design Matthew W. Lluis. More information can be found Communic...

Nitrile Oxide−Dehydroalanine Cycloaddition is a bioconjugation strategy between dehydroalanine-containing proteins and nitrile oxides, remarkably, in fully aqueous-buffered systems without an additional catalyst. This novel method of chemical protein modification forms isoxazoline ring enhancing the diversity function structure. The 1,3-dipolar cycloaddition reaction adds fluorescent label to f...

We describe for the first time, the synthesis and some bioconjugation applications of an original heterotrifunctional cross-linking reagent (also named tripod) bearing three different bioorthogonal functional groups which are fully compatible amongst themselves. Contrary to the first generation tripod recently reported by us (Org. Biomol. Chem., 2008, 6, 3065), the use of an azido group instead...

In a bid to find an efficient means to radiolabel biomolecules under mild conditions for PET imaging, a bifunctional (18)F prosthetic molecule has been developed. The compound, dubbed [(18)F]FPyPEGCBT, consists of a 2-substituted pyridine moiety for [(18)F]F(-) incorporation and a 2-cyanobenzothiazole moiety for coupling to terminal cysteine residues. The two functionalities are separated by a ...

High-throughput generation of bispecific molecules promises to expedite the discovery of new molecular therapeutics and guide engineering of novel multifunctional constructs. However, high synthesis complexity and cost have hampered the discovery of bispecific molecules in drug development and biomedical research. Herein we describe a simple solid-phase bioconjugation procedure for preparation ...

The modification of protein surfaces employing cationic and anionic species enables the assembly of these biomaterials into highly sophisticated hierarchical structures. Such modifications can allow bioconjugates to retain or amplify their functionalities under conditions in which their native structure would be severely compromised. In this work, we assess the effect of this type of bioconjuga...