We develop a riboadenosine-substituted DNA probe-based bioluminescence method for real-time monitoring of exonuclease III (Exo III) activity and Exo III-assisted target recycling. This method employs the target-dependent ATP as the bioluminescence reporter without the involvement of any sophisticated luciferase manipulations, and the glow-type reaction kinetics facilitates the performance of im...

Spectroscopic properties of amino-analogs of luciferin and oxyluciferin were investigated to confirm the color modulation mechanism of firefly (beetle) bioluminescence. Fluorescence solvatochromic character of aminooxyluciferin analogs indicates that the bioluminescence of aminoluciferin is useful for evaluating the polarity of a luciferase active site.

We describe the first direct testing of the antimicrobial susceptibilities of bacterial pathogens in human clinical fluid samples by the use of ATP bioluminescence. We developed an ATP bioluminescence assay that eliminates somatic sources of ATP to selectively quantify the bacterial load in clinical urine specimens with a sensitivity of <1,000 CFU per milliliter. There was a log-log relationshi...

ackground: Histology-based survival assessment of cell grafts does not allow for in vivo follow-up. In this study we introduce two new experimental models for longitudinal in vivo survival studies of cardiac cell grafts using optical bioluminescence imaging. ethods: H9c2 cardiomyoblasts expressing both firefly luciferase (fluc) and green fluorescent protein (GFP) reporter genes were implanted i...

BACKGROUND In vivo bioluminescence, fluorescence, and single-photon emission computed tomography (SPECT) imaging provide complementary information about biological processes. However, to date these signatures are evaluated separately on individual preclinical systems. In this paper, we introduce a fully integrated bioluminescence-fluorescence-SPECT platform. Next to an optimization in logistics...

Tögel F, Yang Y, Zhang P, Hu Z, Westenfelder C. Bioluminescence imaging to monitor the in vivo distribution of administered mesenchymal stem cells in acute kidney injury. Am J Physiol Renal Physiol 295: F315–F321, 2008. First published May 14, 2008; doi:10.1152/ajprenal.00098.2008.—Effective and targeted delivery of cells to injured organs is critical to the development of cell therapies. Howev...

An adenosine-5’-triphosphate (ATP) bioluminescence method was developed for detecting microbial activity in indoor air. This method was compared with the traditional method of collecting, culturing and counting CFUs. A comparison of the results showed that ATP bioluminescence, expressed as RLUs, was moderately correlated with the entire set of CFU counts (r = 0.607), and that the correlation im...

Bioluminescence is widespread throughout the phylum Annelida and occurs in terrestrial marine lineages. Among taxa, bioluminescence has been documented eight families anecdotally reported six additional families. Although new bioluminescent systems have recently described annelids, there are still many other whose light emission mechanisms not sufficiently studied. Some of these include lumines...

effects of atp and mg2+ concentrations on bioluminescence reaction of luciferase (photinus pyralis) were investigated by home-made luminometer. the michaelis constants of the enzyme for atp and mg2+ obtained from the lineweaver-burk graph, were 61.9 mm ±3.3 mm and 251.6mm ± 39.0mm respectively. optimum concentration of mg2+ for maximum luminescence was 0.004 m.