The production of fuel ethanol from low-cost lignocellulosic biomass currently suffers from several limitations. One of them is the presence of inhibitors in lignocellulosic hydrolysates that are released during pre-treatment. These compounds inhibit growth and hamper the production of ethanol, thereby affecting process economics. To delineate the effects of such complex mixtures, we conducted ...

Non-sterile heterotrophic cultivation of Galdieria sulphuraria in presence digestate as well straw after hydrolysis was investigated. G. can be grown pure at rates 0.9 day−1 with glucose. However, a proteolytic treatment resulted increased growth (1.2 day−1) and doubled cell concentrations. Furthermore, utilize glucose obtained hydrolysis. Biomass yields limited cultures were around g per gluco...

Innovative and decentralized biorefineries are needed in urban areas to contribute local resource efficiency. In this case study a biorefinery (waste-to-resource-unit, W2RU) is introduced for bioconversion of food waste using heterotrophic microalgae protein-rich biomass, simultaneous extraction high-value chemicals pigments (e.g., astaxanthin, β-carotene, lycopene, lutein, or riboflavin), vita...

Rice straw hemicellulosic hydrolysate was used as fermentation medium for ethanol production by Pichia stipitis NRRL Y-7124. Shaking bath experiments were initially performed aiming to establish the best initial xylose concentration to be used in this bioconversion process. In the sequence, assays were carried out under different agitation (100 to 200 rpm) and aeration (V(flask)/V(medium) ratio...

Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium, and achieved the highest production rate and yield of hydrogen. Highest H2 yield of 2.02 mol H2/mol-glucose was achieved from 24 h...

Poultry by-products and rainbow trout (Onchorhynchus mykiss) viscera are abundant and underutilized resources that can be used as a unique protein source to make protein hydrolysates. In this study protein hydrolysate were made from these two different sources with Alcalase 2.4L. The functional properties of Fish viscera protein hydrolysate (FPH) compared to poultry by-products protein hydrolys...

The efficient production of biofuels from cellulosic feedstocks will require the efficient fermentation of the sugars in hydrolyzed plant material. Unfortunately, plant hydrolysates also contain many compounds that inhibit microbial growth and fermentation. We used DNA-barcoded mutant libraries to identify genes that are important for hydrolysate tolerance in both Zymomonas mobilis (44 genes) a...

BACKGROUND Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to 'hydrolysate toxicity,' a major problem for microorganisms to achieve complete sugar utilization. Therefor...

Since Yarrowia lipolytica, an oily yeast, contains many valuable products as biomass, to increase its widespread use, it is very important develop a sustainable production model. In this study, the of high acid hydrolysate from sawdust which produced in amounts our environment, and effect these on growth efficiency lipolytica were investigated. A preliminary statistical analysis was carried out...

In this work, microbial lipid production from non-detoxified Eucalyptus bark hydrolysate (EBH) with oleaginous xylose-utilizing Ashbya gossypii strains was explored. The best producing strain a set of engineered identified in synthetic media mimicking the composition EBH (SM), profile characterized, and yeast extract corn steep liquor (CSL) were pinpointed as supplements enabling good balance b...