introduction: despite high level of vaccination against pertussis‚ whooping cough has re-emerged as a health threat, especially in infants. this could be related to expansion of bordetella pertussis with novel alleles for virulence factors including the pertussis toxin promoter, ptxp3. compared to ptxp1 strains‚ ptxp3 strains produce more pertussis toxin which results in immune suppression and ...

The BvgAS phosphorelay regulates ∼10% of the annotated genomes of Bordetella pertussis and Bordetella bronchiseptica and controls their infectious cycles. The hierarchical organization of the regulatory network allows the integration of contextual signals to control all or specific subsets of BvgAS-regulated genes. Here, we characterize a regulatory node involving a type III secretion system (T...

INTRODUCTION Pertussis is a highly contagious disease caused by Bordetella pertussis. It poses a high morbidity and mortality rate, especially among infants younger than 6 months old. In Argentina, pertussis incidence and mortality have increased over the past three decades. OBJETIVE To establish Bordetella pertussisantibody titers among pregnant women in their third trimester and among newbo...

To identify the minimal structures that may be important for the creation of a synthetic and/or recombinant vaccine against whooping cough, human T cell clones were obtained against Bordetella antigens. Cloned peripheral blood T lymphocytes from an immune donor were grown in IL-2 and tested for proliferation in response to inactivated Bordetella species (B. pertussis, B. parapertussis, and B. b...

No standardized PCR method is available for the laboratory diagnosis of the pertussis syndrome. Consensus recommendations for the use of PCR in the diagnosis of Bordetella pertussis infections have been proposed, and the aim of this study was to develop a method that fulfills all of these criteria. A rapid-cycle shared-primer PCR method with a microwell format and probe hybridization detection ...

NOTE. CI, confidence interval; OR, odds ratio. a O145, O111, O103, O26, and the group of the remaining STEC were not statistically significant. 3. Mobberley-Schuman PS, Connelly B, Weiss AA. Phagocytosis of Bordetella pertussis incubated with convalescent serum. J Infect Dis 2003; 187:1646–53. 4. Rodriguez ME, Hellwig SM, van der Pol WL, Hozbor D, Leusen J, van de Winkel JGJ. Fc receptor-mediat...

Background The lack of animal models to experimentally study how infectious agents transmit between hosts limits our understanding of what makes some pathogens so contagious. Methods We recently developed a Bordetella bronchiseptica mouse model to study transmission and have used it to assess, for the first time, which of several well-studied "virulence factors" common to classical Bordetella...