An uncontrolled used of antibiotics and sulphonamides is responsible for the presence of residues in beehive products and contributes to the problem of food safety. The optimisation of analytical methods for the detection of residues of antibacterial drugs (sulphonamides, SPs; tylosin, TL; streptomycin, ST) in honey was pursued and the methods applied to routine samples. ST was investigated by ...

Isocratic high-performance liquid chromatography methods are described for separating the major classes of phospholipids and for isolating the individual molecular species of phospholipids. Fractionation of a total lipid extract of rat liver on a silica column resulted in quantitative recoveries of all major phospholipids with preservation of their fatty acid composition. Rat liver phosphatidyl...

On-line automation of two different liquid chromatographic procedures, a pre-column derivatization system and a pre- and post-column system, in order to generate chemiluminescence is reported. Dansyl chloride (Dns-Cl) was used as a pre-column reagent to form fluorophores and bis(2,4,6-trichlorophenyl) oxalate (TCPO) and hydrogen peroxide (H2O2) as a post-column reagent to generate chemiluminesc...

We describe a liquid-chromatographic procedure for determining nadolol in plasma. After an analog of nadolol is added as internal standard, the plasma sample is passed through a disposable BondElut C18 column. After several column washes, nadolol and the internal standard are eluted with methanol, and the eluate is evaporated and reconstituted with the mobile phase (acetonitrile/water, perchlor...

We demonstrate a liquid-chromatographic method involving post-column derivatization for determining the concentration of thiamin and its phosphate esters in human blood. Blood, erythrocytes, or plasma is deproteinized and centrifuged. Aliquots of the samples are applied to a mu Bondapak C18 column attached to a "high-performance" liquid chromatograph. Addition of potassium ferricyanide/sodium h...

A simple, precise and rapid RP-HPLC method was developed for the determination of ebastine in pharmaceutical formulations. The method was carried out on a Phenomenex RP-C18 column using a mixture of methanol and water (90:10) and detection was done at 262 nm. The linearity range was 5-100 mug/ml. The intra-day and inter-day precision were in the range of 0.22% to 0.49% and 0.24% to 0.73%, respe...

A simple, sensitive isocratic method for the detection and quantification of ciprofloxacin in plasma and urine has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma proteins were removed by a fast and efficient procedure. For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C18 reversed-phase column. T...

A high-pressure liquid chromatographic method for the determination of norfloxacin or ciprofloxacin concentrations in body fluids was developed and compared with a standard bioassay. The high-pressure liquid chromatographic assay utilizes a reverse-phase C18 column, an internal standard, and fluorescence detection, with reproducibility studies yielding coefficients of variation ranging from 0.6...

A simple and rapid HPLC method for the determination of simvastatin using a C18-Hypersil column and acetonitril–phosphate buffer–methanol (5 : 3 : 1, v/v/v) as a mobile phase with detection at 230 nm was proposed. Commercial pharmaceutical tablets were analyzed with a linear range for simvastatin up to 1.884 mg % and a regression coefficient of 0.9995. The method is found to be precise, accurat...

Khuhawar et al. (1998) studied the HPLC determination of rifampicin, isoniazid and pyrazinamide in the pharmaceutical preparations. Isoniazid alone and along with pyrazinamide and rifampicin has been estimated by HPLC. Rifampicin and pyrazinamide were separated from the derivative of isoniazid and are simultaneously determined. The chromatography is carried out from C18 column. These drugs were...