Immobilization of tissue-samples for atomic for microscopy (AFM) is typically done using either semi-dry tissue or by gluing the tissue sample down, both of which can introduce artifacts. Here, we describe the design of a Soft- Clamping Immobilizing Retainer of Tissue (SCIRT) for consistent and nondestructive immobilization of tissues for AFM analysis. We compare the performance of our SCIRT me...

A number of surface modification techniques using immobilization of biofunctional molecules of Titanium (Ti) for dental implants as well as surface properties of Ti and Ti alloys have been developed. The method using passive surface oxide film on titanium takes advantage of the fact that the surface film on Ti consists mainly of amorphous or low-crystalline and non-stoichiometric TiO(2). In ano...

in chemical engineering, several processes are represented by singular boundary value problems. in general, classical numerical methods fail to produce good approximations for the singular boundary value problems. in this paper, chebyshev finite difference (chfd) method and dtm-pad´e method, which is a combination of differential transform method (dtm) and pad´e approximant, are applied for sol...

In real-coded genetic algorithms, some crossover operators do not work well on functions which have their optimum at the corner of the search space. To cope with this problem, we have proposed a boundary extension methods which allows individuals to be located within a limited space beyond the boundary of the search space. In this paper, we give an analysis of the boundary extension methods fro...

The paper presents main results of the investigation of the coupled BEM and FEM applied to a nonlinear generally nonmonotone exterior boundary value problem. The problem consists of a nonlinear diier-ential equation considered in an annular bounded domain and the Laplace equation outside. These equations are equipped with boundary and transmission conditions. The problem is reformulated in a we...

In order to overcome the difficulties of low computational efficiency and high memory requirement in the conventional boundary element method for solving large-scale potential problems, a fast multipole boundary element method for the problems of Laplace equation is presented. through the multipole expansion and local expansion for the basic solution of the kernel function of the Laplace equati...

We analyze adaptive mesh-refining algorithms in the frame of boundary element methods (BEM) and the coupling of finite elements and boundary elements (FEM-BEM). Adaptivity is driven by the two-level error estimator proposed by Ernst P. Stephan, Norbert Heuer, and coworkers in the frame of BEM and FEM-BEM or by the residual error estimator introduced by Birgit Faermann for BEM for weakly-singula...

The boundary knot method (BKM) is a recent boundary-type radial basis function (RBF) collocation scheme for general PDE’s. Like the method of fundamental solution (MFS), the RBF is employed to approximate the inhomogeneous terms via the dual reciprocity principle. Unlike the MFS, the method uses a non-singular general solution instead of a singular fundamental solution to evaluate the homogeneo...

A glucoamylase-immobilized system based on cross-linked gelatin nanoparticles (CLGNs) was prepared by coacervation method. This system exhibited characteristics of temperature-triggered phase transition, which could be used for enzyme immobilization and release. Their morphology and size distribution were examined by transmission electron microscopy and dynamic light scattering particle size an...

The Staudinger ligation between an azido-protein and a phosphinothioester-derivatized surface is demonstrated to be an effective means for the site-specific, covalent immobilization of a protein. Immobilization yields of >50% are obtained in <1 min, and immobilized proteins have >80% of their expected activity. No other method enables more rapid immobilization or a higher yield of active protei...