The equivalent chain length (ECL) has been widely accepted as an identification tool for fatty acid methyl esters (FAMEs). In this study, FAMEs from the seed oil of Chinese mustard (Brassica juncea) analyzed on a 0.25-mm-i.d. capillary column coated with an SA-WAX are used as an example for the calculation of the ECL in single-, two-, three-, and four-step temperature-programmed gas chromatogra...

1. General introduction 2. General consideration in choosing target genes for phytoremediation 3. Genetic engineering of metal transporters 3.1. Uptake of Metal from the Soil to the Root 3.2. Translocation from the Root to the Shoot 3.3. Vacuolar Transporters 3.4. Manipulating Metal Distribution: Some Aspects 4. Genetic engineering of metal chelation 4.1. Glutathione 4.2. Phytochelatin 4.3. Met...

The shape of the leaf is primary phenotype which determines commercial value mustard (Brassica juncea). However, there arefew reports on lobed-leaf gene B. juncea, and molecular regulatory mechanisms underlying margin formation are unknown. In this study, an F2 population derived from ‘MN001’ ‘MU056’ was constructed. Genetic analysis revealed that trait controlled by a major gene, lobed leavesa...

Background: Effects of cadmium (Cd) metal on growth, photosynthetic pigments, level of osmolytes and elements, and antioxidant potential of Brassica juncea seedlings were evaluated. Materials and Methods: Seeds were exposed to different concentrations of metal, i.e., 0, 0.2, 0.4, and 0.6 mM Cd. To investigate the metal influence on biochemical and physiological factors, observations were made o...

Brassica juncea seedlings contained a twofold higher glucosinolate content than B. rapa and these secondary sulfur compounds accounted for up to 30% of the organic sulfur fraction. The glucosinolate content was not affected by H2S and SO2 exposure, demonstrating that these sulfur compounds did not form a sink for excessive atmospheric supplied sulfur. Upon sulfate deprivation, the foliarly abso...

Mitochondrial atpA transcripts were examined in cytoplasmic male sterile (CMS) and fertility restorer lines of CMS (Moricandia arvensis) Brassica juncea. Male sterile flowers had longer atpA transcripts than male fertiles. The mitochondrial atpA region of the CMS line was cloned and sequenced. The 5' and 3' ends of the atpA transcripts of the CMS and the fertility restorer lines were mapped and...

In this study, among a collection of Ni-resistant bacterial strains isolated from the rhizosphere of Alyssum serpyllifolium and Phleum phleoides grown on serpentine soil, five plant growth-promoting bacteria (PGPB) were selected based on their ability to utilize 1-aminocyclopropane-1-carboxylate (ACC) as the sole N source and promote seedling growth. All of the strains tested positive for indol...