Although the Early Soybean Production System (ESPS) in the Midsouthern USA increased seed yield under irrigated and non-irrigated conditions, heat stress and drought still lead to poor seed quality in heat sensitive soybean cultivars. Our breeding goal was to identify breeding lines that possess high germination, nutritional quality, and yield potential under high heat and dryland production co...

Breeding wheat with durable resistance to the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), a major threat cereal production, is challenging due rapid evolution of virulence. Increased durability and broad-spectrum can be achieved by introducing more than one gene, but combining numerous unlinked genes breeding laborious. Here we generate polygenic Pgt transgene cassette five into bre...

Genomic Selection (GS) is a new breeding method in which genome-wide markers are used to predict the breeding value of individuals in a breeding population. GS has been shown to improve breeding efficiency in dairy cattle and several crop plant species, and here we evaluate for the first time its efficacy for breeding inbred lines of rice. We performed a genome-wide association study (GWAS) in ...

Twenty-four cowpea breeding lines and four cultivars were tested for resistance to Meloidogyne incognita in the greenhouse. Gall and egg mass ratings indicated a range of responses from susceptible to highly resistant. Five breeding lines - VS84-2, VS84-8, VS84-12, VS84-14, and VS84-22 - and the cultivar Erectset had gall and egg mass ratings comparable to the M. incognita-resistant cultivar Mi...

Aflatoxin contamination costs the U.S. peanut (Arachis hypogaea L.) industry over $20 million annually. The development of peanut cultivars with resistance to preharvest aflatoxin contamination (PAC) would reduce these costs. Screening techniques have been developed that can measure genetic differences in aflatoxin contamination and they have been used to identify accessions that exhibited rela...

Commercial corn breeding and trait integration have changed modern corn production. In recent years, technology advances for control of insects, weeds, and diseases have improved corn yield levels significantly. Conventional breeding continues to be the foundation of new inbreds (parent lines) for hybrid development. Another method of inbred development is called doubled haploids. This methodol...

We recently reported two novel breeding lines of rats known as Carioca Highand Low-conditioned Freezing (CHF and CLF), based on defensive freezing responses to contextual cues previously associated with electric footshock. The present study used animals of the 8th generation of our selective breeding program to investigate both contextual fear extinction and re-extinction. The results consisten...

Cold stress is one of the major abiotic stresses that impede rice production. A interconnected breeding (IB) population consisted of 497 advanced lines developed using HHZ as the recurrent parent and eight diverse elite indica lines as the donors were used to identify stably expressed QTLs for CT at the booting stage. A total of 41,754 high-quality SNPs were obtained through re-sequencing of th...

Leišová-Svobodová L., Tomková L., Sedláček T., Psota V., Kučera L. (2014): The application of microsatellite analysis in barley malting quality breeding programmes. Czech J. Genet. Plant Breed., 50: 268–277. A set of 43 microsatellite loci was used to characterise the malting barley varieties and breeding lines used for the Protected Geographical Indication (PGI) “České pivo” (“Czech beer”) and...