Systemic enzyme therapy was recently subjected to experimental investigations and to rigorous clinical studies in cancer patients. The designs of the relevant clinical cohort studies followed the guidelines of Good Epidemiological Practice and represent level IIB in evidence-based medicine (EBM). Scientifically sound experimental in vitro and in vivo investigations are far advanced and document...

OBJECTIVE To compare the effectiveness and safety of the triple combination Phlogenzym (rutoside, bromelain, and trypsin) with double combinations, the single substances, and placebo. DESIGN Multinational, multicentre, double blind, randomised, parallel group design with eight groups structured according to a factorial design. SETTING Orthopaedic surgery and emergency departments in 27 Euro...

In this work, bromelain is recovered from triturated pineapple stem and rind (usually kitchen waste) through the precipitation process with alcohol at low temperature. The temperature control is crucial to avoid the irreversible protein denaturation and consequently improving the precipitation yield. The process is carried out in a fed-batch system, so that its dynamic nature poses challenging ...

AIMS AND OBJECTIVES To evaluate the effect of bromelain on pain and swelling after surgical removal of third molars. MATERIALS AND METHODS A prospective randomized clinical study was conducted among 40 patients requiring surgical removal of impacted mandibular third molars. All the impacted molars were surgically removed, and the patients were administered bromelain along with amoxicillin. Pa...

The polyclonal B-cell response to Escherichia coli lipopolysaccharide was studied in C57BL/6 mice maintained after weaning on either a moderate protein-restricted diet with 8% casein or a normal diet. After in vitro or in vivo stimulation with the endotoxin, autoreactive and anti-hapten antibody-producing cells were quantitated by direct plaque assay, using bromelain-treated mouse erythrocytes ...

BACKGROUND K88 positive enterotoxigenic Escherichia coli (K88+ ETEC) is an important cause of diarrhoea in young piglets. K88+ ETEC pathogenesis relies on attachment to specific glycoprotein receptors located on the intestinal mucosa. Proteolytic treatment of these receptors in vitro and in vivo prevents attachment of K88+ ETEC to piglet small intestines and may be of clinical use to prevent K8...

The purpose of this study was to investigate the application of bromelain in the treatment of pain, swelling, and limited mouth opening in patients with hematologic tumor after extraction of the third molar during chemotherapy. We recruited 72 patients with hematologic tumor who were treated by oral and maxillofacial surgery in our hospital, and third molar extraction was performed under local ...

Bromelain is a general name for a family of sulfhydryl that can be found in the proteolytic enzyme group from pineapples (Ananas Comosus). In this study focuses on the prediction of three dimensional structure (3D) of bromelain using homology modelling by MODELLER. The amino acid sequence of bromelain was obtained from the NCBI database and the analysis was done using various bioinformatics too...

A pulsed-cap microcolumn was used for bromelain extraction from pineapple juice by reversed micelles. The cationic micellar solution used BDBAC as the surfactant, isooctane as the solvent and hexanol as the co-solvent. In order to capture the dynamic behavior and the nonlinearities of the column, the operating conditions were modified in accordance with the central composite design for the expe...

A 59-year-old man was admitted to our department with epigastric pain and nausea. The patient mentioned that he had swallowed heated mastic to treat constipation shortly before his symptoms developed. He had no history of any diseases. His vital signs were within normal limits. The physical examination findings were normal, and the results of laboratory tests were unremarkable. Upper gastrointe...