Receptor-mediated changes in cytoplasmic calcium concentrations occur either through release from intracellular calcium stores or by the opening of channels in the plasma membrane, allowing influx of calcium from the extracellular fluid. Carbachol, a muscarinic receptor agonist, stimulated both calcium influx and inositol 1,4,5-trisphosphate (InsP3)-mediated intracellular calcium release in A9 ...

Because of an elevated serum phosphate level, patients who suffer from chronic kidney failure frequently tend to have cardiovascular calcification and are therefore exposed to a higher probability of a fatal event. Phosphate binders are able to reduce these negative effects. Currently, there are primarily two groups of phosphate binders (calcium-containing and calcium-free phosphate binders) wh...

We have previously reported that Zn(2+) infused into the coronary arteries of isolated rat hearts leads to the potent dephosphorylation of phospholamban (PLB) as well as a noticeable but less potent dephosphorylation of the ryanodine receptor 2. We hypothesized in the present study that a Zn(2+)-activated phosphatase is located in the vicinity of the sarcoplasmic reticulum (SR) where PLB and ry...

The interaction force between a rigid silica sphere and a butyl or octyl acetate droplet was measured in an aqueous environment using atomic force microscopy (AFM). The force measurements were performed without added stabilizers and the observed force behavior was found to be dependent on the type of inorganic electrolyte present, where the interfacial tension was constant over the electrolyte ...

Introduction. Due to chronic inflammation, maintenance hemodialysis (MHD) patients continue show excess mortality. Acetate-free citrate-buffered A concentrates could be a way improve the biocompatibility of procedure, reduce and thus in long term prognosis patients. Methods. Using pre-post design (3 months acetate followed by 3 citrate-acidified standard bicarbonate-based dialysate hemodialysis...

Protein kinase capable of phosphorylating 40S ribosomal protein S6 on serine residues has been detected in chicken embryo fibroblasts. This activity appears to be regulated in direct response to expression of pp60v-src in chicken embryo fibroblasts infected with a temperature-sensitive transformation mutant of Rous sarcoma virus. Partially purified S6 kinase was highly specific for S6 in 40S ri...

GUROFF, GORDON, HECTOR F. DELUCA, AND HARRY STEENBOCK. Citrate and action of vitamin D on calcium and phosphow metabolism. Am. J. Physiol. 20&J: 833-836. 1c~63.-Pantothenic acid and pyridoxine deficiencies in rats greatly minimized the rise in serum citrate following vitamin D administration without affecting the rise in serum calcium, serum phosphorus, and femur ash. In addition, pyridoxine de...

Mo3e is a protein (p 50,80) that is expressed on the surface of human monocytic cells after exposure in vitro to soluble activating factors that include bacterial lipopolysaccharide, muramyl dipeptide, and phorbol myristate acetate (PMA). The surface expression of Mo3e may represent a cellular event that occurs in response to the formation of "secondary messengers" that include diacylglycerol, ...

Human Jurkat T cells containing a stably integrated human T cell leukaemia virus type 1 (HTLV-1) long terminal repeat (LTR) reporter gene construct were used to study the role of calcium-dependent cellular activation pathways in LTR trans-activation. Treatment of these cells with the calcium ionophore ionomycin resulted in a reduced basal response of the LTR and reduced responses to 12-O-tetrad...

In previous communications we have reported the experimental production of encephalomyelitis in guinea pigs with some brain lipid extracts and brain residue (after lipid extraction with cold acetone, ether and alcohol), composed mostly of proteins (1). In the present report we shall record our observations on the production of experimental encephalomyelitis in guinea pigs with a protein calcium...