Procedures were developed for the purification of calmodulin from the eggs and from the sperm of the sea urchin Strongylocentrotus purpuratus and Arbacia punctulata. Two forms of calmodulin were isolated from A. punctulata eggs, one from each of the other three sources. All five calmodulins are similar to vertebrate calmodulin as judged by their activation of bovine brain cyclic nucleotide phos...

Aluminum (Al) toxicity imposes a significant limitation to crop production in South Western Australia. This paper examines the impact of surface-applied lime and gypsum on soil solution chemistry short term (1 year) long-term (10 years) water limited environments. In experiments, we measured using paste extract profile samples collected depth 50 cm. We then used chemical equilibrium model MINTE...

Chondroitinase B from Pedobacter heparinus is the only known enzyme strictly specific for dermatan sulfate and is a widely used enzymatic tool for the structural characterization of glycosaminoglycans. This beta-helical polysaccharide lyase belongs to family PL-6 and cleaves the beta(1,4) linkage of dermatan sulfate in a random manner, yielding 4,5-unsaturated dermatan sulfate disaccharides as ...

BACKGROUND Smaller nanoparticles facilitate the delivery of DNA into cells through endocytosis and improve transfection efficiency. The aim of this study was to determine whether protamine sulfate-coated calcium phosphate (PS-CaP) could stabilize particle size and enhance transfection efficiency. METHODS pEGFP-C1 green fluorescence protein was employed as an indicator of transfection efficien...

The thermophilic Geobacillus bacterium catalyzed the formation of 100-μm hexagonal crystals at 60°C in a hydrogel containing sodium acetate, calcium chloride, and magnesium sulfate. Under fluorescence microscopy, crystals fluoresced upon excitation at 365 ± 5, 480 ± 20, or 545 ± 15 nm. X-ray diffraction indicated that the crystals were magnesium-calcite in calcite-type calcium carbonate.

BACKGROUND Multiple treatment modalities exist for unicameral bone cysts (UBC), including steroid injection, autologous bone marrow injection, mechanical decompression, intramedullary fixation, curettage, and bone grafting. All have their own potential limitations such as high recurrence rates, cyst persistence, need for multiple procedures, and prolonged immobilization. A minimally invasive re...