A physiologically significant level of intracellular carbonic anhydrase has been identified in Chlamydomonas reinhardtii after lysis of the cell wall-less mutant, cw15, and two intracellular polypeptides have been identified which bind to anti-carbonic anhydrase antisera. The susceptibility of the intracellular activity to sulfonamide carbonic anhydrase inhibitors is more than three orders-of-m...

We have examined the induction of carbonic anhydrase activity in Chlamydomonas reinhardtii and have identified the polypeptide responsible for this activity. This polypeptide was not synthesized when the alga was grown photoautotrophically on 5% CO(2), but its synthesis was induced under low concentrations of CO(2) (air levels of CO(2)). In CW-15, a mutant of C. reinhardtii which lacks a cell w...

1. Carbonic anhydrase activity in 'membrane' and 'cytosol' fractions of goldfish erythrocytes was assayed by the p-nitrophenyl acetate procedure following thermal acclimation. 2. The thermal sensitivity of "membrane"-associated activity was apparently unaltered by acclimation. "Cytosol" activity in warm-acclimated specimens was somewhat more thermosensitive than that animals maintained at low t...

Natural products (NPs) have proven to be an invaluable source of new chemotherapies yet very few have been explored to source small molecule carbonic anhydrase (CA) inhibitors. CA enzymes underpin physiological pH and are critical to the progression of several diseases including cancer. The present study is the first to more widely investigate NP coumarins for CA inhibition following the recent...

The temperature dependence of the activity and structure of the enzyme carbonic anhydrase was studied. The Arrhenius plot shows a jump which is seen usually in proteins with more than one subunit or with one subunit but more than one domain. Since carbonic anhydrase has only one subunit with one domain, the fine conformational changes of the protein motifs could only be detected through circula...

Acetobacterium woodii, Acetohalobium arabaticum, Clostridium formicoaceticum, and Sporomusa silvacetica were found to contain carbonic anhydrase (CA). Minimal to no CA activity was detected in Moorella thermoautotrophica, Moorella thermoacetica subsp. "pratumsolum," Sporomusa termitida, and Thermoanaerobacter kivui. Of the acetogens tested, A. woodii had the highest CA specific activity, approx...

S. ALIAKBAR,*t P. R. BROWN,* E. JAUNIAUX,t D. E. BIDWELLS and K. H. NlCOLAIDESt *Department of Biochemistry, King’s College London, Strand, London WC2R 2L.S. tllarris Birthright Research Centre for Fetal Medicine, Department of Obstetrics and Gynaecology, King’s College Hospital, Denmark Hill, London SE5 8RX, and $ Nufield Laboratories of Comparative Medicine, The Zoological Society of London, ...

This study uses mutants of human carbonic anhydrase (HCAII) to examine how changes in the organization of water within a binding pocket can alter the thermodynamics of protein-ligand association. Results from calorimetric, crystallographic, and theoretical analyses suggest that most mutations strengthen networks of water-mediated hydrogen bonds and reduce binding affinity by increasing the enth...

Investigations of the cattle karyotypes in the progeny groups of sires, heterozygous for a translocation of centric fusion type and back-crossed to normal dams, showed a deficient number of heterozygotes. As the ratio in the calves was approximately 1: 1 the deficiency in the total back-cross progeny, significant on the 95 per cent level, might have been due to selection against sexually mature...

Two electrophoretic forms of erythrocytic carbonic anhydrase were found to be controlled by one autosomal locus with two codominant alleles, CA(f) and CA(8). The gene frequencies for the CA(f) and CA(8) alleles were found to be.15 and.85, respectively, in a sample of 53 mice from Middlesex County, New Jersey. The observed genotypic frequencies indicated that the population was in Hardy-Weinberg...