An electron microscopical and biochemical study of the chorionic epithelium from 16and 17-day-old chick embryos was conducted. Using a combination of sections made either parallel or perpendicular to the chorionic surface, it was confirmed that the cell populations of the polar and equatorial regions of the membrane are different; the former contains a very reduced number of VC cells which are,...

The airway-surface liquid pH (pHASL ) is slightly acidic relative to the plasma and becomes more acidic in airway diseases, leading to impaired host defense. CO2 in the large airways decreases during inspiration (0.04% CO2 ) and increases during expiration (5% CO2 ). Thus, we hypothesized that pHASL would fluctuate during the respiratory cycle. We measured pHASL on cultures of airway epithelia ...

Human carbonic anhydrase B and C have been purified in high yields by affinity chromatography, using two different affinity gels, incorporating the potent benzenesulfonamide inhibitor, but coupled to Sepharose through different ,spacer-arms~. Although both affinity gels bind carbonic anhydrase B and C quantitatively, they exhibit different binding capacities. Sepharose-glycyi-L-tyrosine-azobenz...

The sequence of amino acid residues comprising the major form of sheep red cell carbonic anhydrase C has been determined. The primary sequences of peptides derived from cyanogen bromide cleavage and tryptic digestion were obtained primarily through the use of the Edman degradation procedure. The ordering of these peptides in the sheep molecule is based on the high degree of homology between the...

The apparent proportion of column-chromatographically measured glycosylated hemoglobin in erythrocytes from an individual with hemoglobins A, S, and G was only 2.3% because the slow glycosylated variant hemoglobins were retarded in the column. In contrast, the value for glycosylated hemoglobin was 7.8% by a new cellulose acetate electrophoretic method that includes use of dextran sulfate buffer...

I N A RECENT REPORT Lie-Injo and 1 described a condition char.. acterized by a deficiency of both types I and 112 (also designated B and C) of carbonic anhydrase. This deficiency, though associated with a high level of fetal hemoglobin, was not due to hemoglobinopathy, hereditary persistence of fetal hemoglobin, or any of the known types of thalassemia. Because of lack of proof of hereditary ba...

Enhanced receptor selectivity: carbonic anhydrase inhibitors are relevant for both cancer diagnosis and therapy. Combining non-radioactive Re compounds with their radioactive (99m)Tc homologs enables the use of identical molecules for therapy and imaging (theragnostic). The syntheses and in vitro evaluation of [(Cp-R)M(CO)(3)] (Cp=cyclopentadienyl, M=Re, (99m)Tc) with R being a highly potent ca...

This study was made to explore the effects of acetazolamide on tumor growth, metastasis and the possible mechanisms. The mice bearing with Lewis lung carcinomas were taken as the animal model. The effects of acetazolamide were compared with the combination treatment of NaHCO3 on tumor growth, metastasis and carbonic anhydrase activity in lung and tumor tissues using imidazole-Tris technique. An...

Methods for purifying carbonic anhydrase (EC 4.2.1.1.) isozymes, carbonic anhydrase B (or I) and C (or IIL by affinity chromatography have been described by Falkbring et al. (1) and Whitney (2), in which affinity gels are formed by coupling, respectively, p-aminobenzenesulfonamide, and p-(aminomethyl)benzenesulfonamide to Sepharose polysaccharides by means of cyanogen bromide activation. Attemp...