Until recently there have been only two well recognized members of the urobilinogen group, mesobilirubinogen, the chromogen of i-urobilin,’ and stercobilinogen, the leuco compound of urobilin or stercobilin. While mesobilirubinogen is readily obtained in crystalline form following amalgam reduction of bilirubin or i-urobilin, stercobilinogen has never been crystallized. Stercobilin, however, wa...

Pigment in animal tissue results from the union of 2 substances: a colorless chromogen and a ferment or enzyme which activates the chromogen. Where either of these is absent there is no pigment, or, if present but in insufficient amount, the tissue is lacking in pigment in a proportionate degree. Lack of pigment in tissue is known as albinism. It is a hereditary genetic defect. The entire body ...

Paper chromatographic techniques have been reported for the separation and identification of steroid compounds related to progesterone and testosterone, in which impregnated filter paper (l-4) and steroid complexes (5, 6) are utilized. After preliminary investigations on the separation of progesterone and its metabolites (7), however, it became apparent that progesterone-like Czl compounds from...

A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated. A surfactant present in the reagent causes the lysis of cells in the sample. Glutathione, ascorbic acid, protein thiols and other main antioxidant substances in the sample react with a specific redox chromogen (2,6-dichlorophenolindophenol) and produce a change in the col...

A simple, rapid and sensitive spectrophotometric method has been developed for the assay of ceterizine hydrochloride (CTZH) in bulk drug and its pharmaceutical preparations. This method is based on the ion-pair complex reaction between CTZH and Alizarin Red S in Clarks-Lubs buffer. The chromogen being extractable with chloroform, could be measured quantitatively at 440 nm. All variables were st...

The classical method to determine hydrogen peroxide (H2O2) scavenging activity of plant extracts is evaluated by measuring the disappearance of H2O2 at a wavelength of 230 nm. Since this method suffers from the interference of phenolics having strong absorption in the UV region, a simple and rapid colorimetric assay was developed where plant extracts are introduced to H2O2, phenol and 4-aminoan...

A new method for characterizing amniotic fluid is presented. The difference in optical absorbarice between amniotic fluid diluted with distilled water or glycerol is measured. This differential approach is shown to determine the total light scattered by the surfactant-containing lamellar bodies and not to be affected by interfering chromogens. The lamellar body size, number density, and concent...

The present work describes two new spectrophotometric methods for the determination of bromhexine hydrochloride in bulk and formulations. Method A is based on the reduction of Fe into Fe by the drug followed by the complex formation of Fe with 2, 2’ bipyridyl to form cherry red colored chromogen having maximum absorbance at 510nm and Method B is based on oxidation of 3-methylbenzothiazolinone-2...

Four simple, sensitive, accurate, and rapid visible spectrophotometric methods (A, B, C and D) have been developed for the estimation of sulfamethoxazole in pharmaceutical Preparation. They are based on the diazotization of sulfamethoxazole with sodium nitrite and hydrochloric acid followed by coupling with N-(1-naphthyl ethylenediamine dihydrochloride (Method A) to form pink coloured chromogen...