CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. Here we found that homeodomain-interacting protein kinase 2 (HIPK2), a DNA-damage respon...

Two conserved histidine residues (His-101 and His-271) appear to be essential components in the active site of the enzyme xylose (glucose) isomerase (EC 5.3.1.5). These amino acid residues were targeted for mutagenesis on the basis of sequence homology among xylose isomerases isolated from Escherichia coli, Bacillus subtilis, Ampullariella sp. strain 3876, and Streptomyces violaceus-niger. Each...

Abstract The results of the study showed two genetic polymorphisms BM4505 marker for studied samples: 270/260, 280/271 bp, their distribution ratio was 60.34 and 39.66 %, respectively, which highly significant differences (P≤0.01) among polymorphisms. that there were (P≤0.05) between 270/260,280/271 bp by California mastitis test, cows carrying 270/260 where test value 1.20 on 2.47, as other tr...

Root-knot nematodes (RKNs) infect many annual and perennial crops and are the most devastating soil-born pests in vineyards. To develop a biotech-based solution for controlling RKNs in grapes, we evaluated the efficacy of plant-derived RNA interference (RNAi) silencing of a conserved RKN effector gene, 16D10, for nematode resistance in transgenic grape hairy roots. Two hairpin-based silencing c...

Rheumatoid arthritis (RA) is a T cell-mediated autoimmune disease, but target antigens (autoantigens) responsible for T cell activation remain unclear. Type II collagen (CII) is a candidate autoantigen that is largely confined to the articular cartilage. To investigate whether CII is an important antigen in patients with RA, we examined peripheral blood T cell reactivity to CII in HLA-DRB1*0101...

Reviews of information CD-ROM products were examined in relation to those criteria which home users identify as important in the selection of information CD-ROMs. Specifically we were interested in identifying what relationship, if any, existed between the key overall elements which characterise the reviews and the medium (print or electronic) and type of publication in which they appeared. A c...

This article emphasises the importance of natural gestures and describes the framework and the development process of the “Signs Workshop” CD-ROM, which is a multimedia application for the promotion of early communication skills of children with developmental disabilities. Signs Workshop CD-ROM was created in the scope of Down’s Comm Project, which was financed by the Calouste Gulbenkian Founda...

The interferon-induced, dsRNA-activated human protein kinase (PKR) exerts antiviral and antiproliferative effects through inhibition of protein synthesis. Studies of structure-function relationships in PKR have shown that two dsRNA binding motifs are important for its autophosphorylation and activation by dsRNA in vitro. To correlate these findings with the activity of PKR in vivo, we examined ...