Red cell oxygen affinity, red cell nucleoside triphosphate (NTP) levels and blood oxygen-carrying capacity were determined for male, nonpregnant and pregnant female, and fetal garter snakes Thamnophis elegans exposed to hypoxia (5 % oxygen) and hyperoxia (100 % oxygen). Male and nonpregnant female snakes were maintained under these conditions for up to 3 weeks and exhibited an apparent maximal ...

Apelin is the endogenous ligand for the APJ receptor, and apelin and APJ are expressed in the gastrointestinal (GI) tract. Intestinal inflammation increases intestinal hypoxia-inducible factor (HIF) and apelin expression. Hypoxia and inflammation are closely linked cellular insults. The purpose of these studies was to investigate the influence of hypoxia on enteric apelin expression. Exposure o...

Leptin and hypoxia are pro-fibrotic factors involved in fibrogenesis, however, the gene expression profiles remain to be fully elucidated. The aim of the present study was to investigate the regulatory roles of leptin and hypoxia on the L929 mouse fibroblast cell line. The cells were assigned to a normoxia, normoxia with leptin, hypoxia, and hypoxia with leptin group. The cDNA expression was de...

Platoshyn O, Yu Y, Ko EA, Remillard CV, Yuan JX. Heterogeneity of hypoxia-mediated decrease in IK(V) and increase in [Ca ]cyt in pulmonary artery smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 293: L402–L416, 2007. First published May 25, 2007; doi:10.1152/ajplung.00391.2006.—Hypoxic pulmonary vasoconstriction is caused by a rise in cytosolic Ca ([Ca ]cyt) in pulmonary artery smooth mu...

B10 is a glycosylated derivative of betulinic acid with promising activity against glioma cells. Lysosomal cell death pathways appear to be essential for its cytotoxicity. We investigated the influence of hypoxia, nutrient deprivation and current standard therapies on B10 cytotoxicity. The human glioma cell lines LN-308 and LNT-229 were exposed to B10 alone or together with irradiation, temozol...

BACKGROUND: Hypoxia adaptation in cancer cells is mediated by hypoxia inducible factor (HIF)-1 that induces the expression of various proteins. Up to date, analysis T47D breast cells-secreted protein still limited. The aim this study was compare profile secreted under hypoxic and normoxic conditions identify proteins as candidate for marker cells.METHODS: were cultured standard conditions. Cell...

Introduction Neuronal cell death is an important factor in the pathogenesis of acute high-altitude cerebral hypoxia; however, underlying molecular mechanism remains unclear. In this study, we tested if hypoxia (HAH) causes neuronal and mitochondrial dysfunction using various vivo vitro approaches. Methods Acute was induced by hypobaric chamber male mice. explored mechanisms immunofluorescence, ...

Background . Glioblastoma multiforme (GBM) develops in the hypoxic microenvironment, which plays an important role pathogenesis of disease and is closely associated with tumor growth, development poor prognosis. Hypoxia increases resistance cells (TC) to radiation therapy chemotherapy, promotes appearance aggressive TC phenotype, leading recurrence. The molecular mechanism action on secretome G...

AIM To investigate if hypoxia induces vascular endothelial growth factor (VEGF)-A and VEGF-C secretion in non-small cell lung cancer (NSCLC) cells and if the secretion is cell type-dependent. MATERIALS AND METHODS Adenocarcinoma (AC) (H522, PAC) and squamous cell carcinoma (SCC) (H520) cell lines were exposed to hypoxia and normoxia. Supernatants were analysed with enzyme-linked immunosorbent...

Cellular prion protein (PrPC) can replace other pivotal molecules due to its interaction with several partners in performing a variety of important biological functions that may differ between embryonic and mature stem cells. Recent studies have revealed major advances in elucidating the putative role of PrPC in the regulation of stem cells and its application in stem cell therapy. What is spec...