A wide variety of biological processes rely upon interactions between proteins and lipids, ranging from molecular transport to the organization of the cell membrane. It was recently established that electrospray ionization mass spectrometry (ESI-MS) is capable of capturing transient interactions between membrane proteins and their lipid environment, and a detailed understanding of the underlyin...

Lipids in eukaryotic cell membranes have been shown to cluster in "rafts" with different lipid/protein compositions and molecular packing. Model membranes such as giant unilamellar vesicles (GUVs) provide a key system to elucidate the physical mechanisms of raft assembly. Despite the large amount of work devoted to the detection and characterization of rafts, one of the most important pieces of...

The influenza virus (IFV) acquires its envelope by budding from host cell plasma membranes. Using quantitative shotgun mass spectrometry, we determined the lipidomes of the host Madin-Darby canine kidney cell, its apical membrane, and the IFV budding from it. We found the apical membrane to be enriched in sphingolipids (SPs) and cholesterol, whereas glycerophospholipids were reduced, and storag...

Cell surface membrane proteins and lipids engage underlying actin filaments in complicated ways. The players engaged are known, and it is clear that the terms of their engagement are important for membrane functions such as endocytosis and receptor-mediated signaling. However, little is known about the way membrane bilayer and membrane skeleton mutually influence one another in these functions....

Atomistic molecular dynamics simulations were performed with 13 non-peptidic neurotransmitters (NTs) in three different membrane environments. The results provide compelling evidence that NTs are divided into membrane-binding and membrane-nonbinding molecules. NTs adhere to the postsynaptic membrane surface whenever the ligand-binding sites of their synaptic receptors are buried in the lipid bi...

Abstract This paper evaluates the pathological condition of Belarusian forests with use monitoring traditional forest factors and remote sensing data. The aim research was to assess pine monitor degradation based on biochemical analyzes needle samples aviation data detection. shooting carried out quasi-synchronously ground sampling needles using an unmanned aircraft complex type. Based results ...

Abstract Objectives: Extracellular vesicles (EVs) are heterogeneous membrane in diameter of 30-5000 nm, that transport proteins, non-coding RNAs (miRNAs), lipids and metabolites. Major populations include exosomes, ectosomes apoptotic bodies. The purpose this study was to compare the distribution EVs obtained under different conditions differential centrifugation, including ultracentrifugation,...

Membranes constitute a meeting point for lipids and proteins. Not only do they define the entity of cells and cytosolic organelles but they also display a wide variety of important functions previously ascribed to the activity of proteins alone. Indeed, lipids have commonly been considered a mere support for the transient or permanent association of membrane proteins, while acting as a selectiv...

Human pluripotent stem cells (hPSCs) are ideal “raw materials” for making various human cell types regenerative medicine and needed in large numbers. 3D suspension culturing (e.g., stirred-tank bioreactor or STR), which suspends cultures an agitated medium, has been extensively studied to scale up hPSC production. However, a significant problem with is the uncontrolled spheroid agglomeration. I...