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A fundamental problem of cell biology and biochemistry is the elucidation of the mechanisms by which the specific components of subcellular membranes are assembled into mature organelles. The major components of all cell membranes are lipids and proteins. The presence of discrete structural motifs contained in the primary sequence of proteins directs a large number of post-translational process...

Traditional Transwell inserts with track-etched 10 ?m thick polymer membranes have been intensively used for studying cellular barriers. However, their thickness hampers direct cell-cell interaction between the adjacent cells which has shown to critically influence barrier formation. Therefore, here effect of reduced distance by using fivefold thinner (2 ?m) optically transparent polydimethylsi...