BACKGROUND Recovering individual genomes from metagenomic datasets allows access to uncultivated microbial populations that may have important roles in natural and engineered ecosystems. Understanding the roles of these uncultivated populations has broad application in ecology, evolution, biotechnology and medicine. Accurate binning of assembled metagenomic sequences is an essential step in rec...

The microbial conversion of lignocellulosic biomass for biofuel production represents a renewable alternative to fossil fuels. However, the discovery of new microbial enzymes with high activity is critical for improving biomass conversion processes. While attempts to identify superior lignocellulose-degrading enzymes have focused predominantly on the animal gut, biomass-degrading communities in...

BACKGROUND Cellulose-degrading thermophilic anaerobic bacterium as a suitable host for consolidated bioprocessing (CBP) has been proposed as an economically suited platform for the production of second-generation biofuels. To recognize the overall objective of CBP, fermentation using co-culture of different cellulolytic and sugar-fermenting thermophilic anaerobic bacteria has been widely studie...

BACKGROUND The diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass to methane. Compared to natural systems, biogas reactors are limited in their hydrolytic potential. The reasons for this are not understood. RESULTS In this paper, we show that a typical industrial biogas reactor fed with maize silage,...

BACKGROUND The recombinant yeast strains displaying the heterologous cellulolytic enzymes on the cell surface using the glycosylphosphatidylinositol (GPI) anchoring system are considered promising biocatalysts for direct conversion of lignocellulosic materials to ethanol. However, the cellulolytic activities of the conventional cellulase-displaying yeast strains are insufficient for the hydroly...

the effect of phosphorus supplementation to ammoniated rice straw was studied. the in vitro experiment was carried out following the first stage of tilley and terry method. the treatments consisting of four diets were a= 50% ammoniated rice straw + 50% concentrate (control), b= a + 0.2% phosphorus (p) supplement, c= a + 0.4% phosphorus (p) supplement, and d= a + 0.6% phosphorus (p) supplement o...

Restoration and optimisation of soil processes is an important task modern agriculture one the reserves for increasing agricultural production. Under current conditions, this becomes possible with integrated introduction ecologised fertiliser systems. The purpose study to investigate regularities development biological features grey forest soil, interdependencies between them, productivity wint...