We investigated the role of intracellular calcium concentration ([Ca2+]i) in endothelin-1 (ET-1) production, the effects of potential vasospastic agents on [Ca2+]i, and the presence of L-type voltage-dependent Ca2+ channels in cerebral microvascular endothelial cells. Primary cultures of endothelial cells isolated from piglet cerebral microvessels were used. Confluent cells were exposed to eith...

Intravascular papillary endothelial hyperplasia (IPEH) is an uncommon benign vascular disease characterized by endothelial cell proliferation and papillary formation within the lumen of blood vessels arising from an organizing thrombus. The occurrence of this uncommon lesion is about 2% of all vascular tumors. IPEH mostly occurs in the 5th decade of life, and there is no gender or age predilect...

Endothelial-to-mesenchymal transition (EndoMT) is a common phenomenon in vascular diseases, while the role of endothelial dysfunction central disease remains to be further investigated. MiR-122 an inflammation-associated non-coding RNA that participates multiple human disease, but whether miR-122 plays as critical EndoMT induced by ischaemic stroke unknown. Although BAI2 known brain-specific in...

Accumulating evidence indicates that aldosterone plays a critical role in the mediation of oxidative stress and vascular damage. NADPH oxidase has been recognized as a major source of oxidative stress in vasculature. However, the relation between NADPH oxidase in aldosterone-mediated oxidative stress in endothelial cells remains to be ascertained. The present study aimed to investigate the rel...

BACKGROUND Vascular endothelial growth factor-B (VEGF-B) protects against experimental stroke, but the effect of stroke on VEGF-B expression is uncertain. METHODS We examined VEGF-B expression by immunohistochemistry in the ischemic border zone 1-7 days after middle cerebral artery occlusion in rats. RESULTS VEGF-B immunoreactivity in the border zone was increased after middle cerebral arte...

The unique component of the cerebral circulation is the so called blood-brain barrier (BBB). The anatomical structures of the BBB consist of the cerebral vascular endothelial cells, the surrounding pericytes, the basal lamina and the perivascular astrocytes. These form the so-called neurovascular unit. Notably, the endothelial cells are interconnected by tight junctions; thus, any solute transp...

objective(s): the aim of this study was to estimate effects of hyperbaric (hb) treatment by determination of cd15s and cd11b leukocyte proinflammatory markers expression.  in addition, this study describes changes in cd77 and cd34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure. materials and methods:expression of cd11b and cd...

Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21...

The blood-brain barrier (BBB) is a complex organization of cerebral endothelial cells (CEC), pericytes and their basal lamina, which are surrounded and supported by astrocytes and perivascular macrophages. Collectively these cells separate and form the compartments of the cerebral vascular space and the cerebral interstitium under normal conditions. Without the BBB, the 'interior milieu' of the...

spinal cord injury (sci) actuate to complex cellular and molecular interactions within the central nervous system in a heave to repair the initial tissue damage. the pathophysiology of acute spinal cord injury (sci) involves primary and secondary mechanisms. neuroinflammation is an important secondary injury process in sci. the local inflammatory microenvironment within the injured spinal cord ...