Human amniotic epithelial cells (hAEC) have stem cell-like features and immunomodulatory properties. Here we show that hAEC significantly suppressed splenocyte proliferation in vitro and potently attenuated a mouse model of multiple sclerosis (MS). Central nervous system (CNS) CD3(+) T cell and F4/80(+) monocyte/macrophage infiltration and demyelination were significantly reduced with hAEC trea...

3. Maibach H.J., Johnson H.L. Contact urticaria syndrome. Contact urticaria to diethyltoluamide. Arch Dermatol 1975;111:726-730. 4. Miller JD. Anaphylaxis associated with insect repellent. N Engl J Med. 1982;307(21):1341—2. 5. Von Mayenburg J, Rakoski J. Contact urticaria to diethyltoluamide. Contact Dermatitis. 1983;9(2):171. 6. Vozmediano JM, Armario J, Gonzalez-Cabrerizo A. Immunologic conta...

Regulatory T cells preserve tolerance to peripheral self-Ags and may control the response to allogeneic tissues to promote transplantation tolerance. Although prior studies have demonstrated prolonged allograft survival in the presence of regulatory T cells (T-reg), data documenting the capacity of these cells to promote tolerance in immunocompetent transplant models are lacking, and the mechan...

Antigen-specific killing ability of effector CD8+ T cells is critical for protective immunity against infection. Here, we describe in vivo cytotoxic T cell assay to examine effector function of antigen-specific CD8+ T cells. Mice infected with Listeria monocytogenes (L. monocytogenes) expressing chicken ovalbumin as a model antigen mount ovalbumin-specific CD8+ T cell responses. Effector CD8+ T...

The effects of TGF-β on dendritic cells (DCs) on the tumor microenvironment are not well understood. We report, here, the establishment of an in vitro lung cancer microenvironment by co-incubation of seminaphtharhodafluor (SNARF) labeled Lewis lung cancer (LLC) cells, carboxyfluorescein succinimidyl ester (CFSE) labeled fibroblasts and 4-chloromet...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 209 No. 1 143–162 www.jcb.org/cgi/doi/10.1083/jcb.201407130 JCB 143 Correspondence to Chang-Duk Jun: [email protected] Abbreviations used in this paper: ABM, actin-binding motif; APB, actin polymerization buffer; APC, antigen-presenting cell; Arp2/3, Actin-related protein 2/3; CFSE, carboxyfluorescein succinimidyl ester; CH, calponin hom...

Chronic myeloid leukemia (CML) stem and progenitor cells overexpress BcrAbl and are insensitive to imatinib mesylate (IM). We therefore investigated whether these cells were efficiently targeted by nilotinib. In K562, the inhibitory concentration (IC50) of nilotinib was 30 nM versus 600 nM for IM, consistent with its reported 20-fold-higher potency. However, in primary CD34(+) CML cells, niloti...

The immune response is a concerted dynamic multi-cellular process. Upon infection, the dynamics of lymphocyte populations are an aggregate of molecular processes that determine the activation, division, and longevity of individual cells. The timing of these single-cell processes is remarkably widely distributed with some cells undergoing their third division while others undergo their first. Hi...