methods and materials: hepatitis b virus dna was detected by conventional pcr in some of the serum samples obtained from hbsag-positive chronic hepatitis b (chb) patients who were referred to health centers of shahrekord for routine monitoring of the disease. subsequently, using real-time pcr, the dna samples were used for genotyping and analysis of resistance to lamivudine. results: the dna wa...

background cryptosporidium spp. is a widespread protozoan parasite involving humans and animals. objectives this study was carried out to evaluate the immunofluorescence assay (ifa) and pcr results for more accurate diagnosis of faecal specimens. patients and methods forty six faecal human specimens of cryptosporidium oocysts were examined by pcr and ifa in calgary, canada. in statistical analy...

background toxoplasma gondii is an opportunistic parasitic organism causing infection in many mammals, including immunosuppressed patients. toxoplasmosis as an opportunistic infection is highly prevalent among patients receiving a kidney transplant. objectives the purpose of this study was to identify and determine the prevalence of toxoplasma gondii in clinical samples collected from patients ...

background and aims: sexually transmitted infections (stis) are of major concern to clinicians and researchers in the field of reproductive medicine. many sti pathogens cause incurable and often fatal diseases, and have been transmitted through insemination procedures. the role of herpes simplex virus in male infertility has been investigated using the sensitive methods. the aim of this study w...

conclusions the high prevalence of oxa β-lactamase and high genetic diversity of p. aeruginosa indicated that the resistance of p. aeruginosa might be expanding in our studied hospitals. background pseudomonas aeruginosa is main bacterial pathogen accountable for nosocomial infections. furthermore, it could potentially become resistant to β-lactams, aminoglycosides and fluoroquinolones antibiot...

chlamydia pneumoniae is a common respiratory pathogen which is often found in our paediatric populations. many patients with community-acquired pneumonia caused by c. pneumoniae have symptoms suggestive of sinusitis.the role of c. pneumonia in rhinosinusiits children (meanage =4.3 ±2.5year). this case control study was done in the pediatric and ent clinics of hazrat rasul hospital in tehran (20...

although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this stu...

background and objective: human papillomavirus (hpv) is one of the possible etiologic factors  in development of esophageal squamous cell carcinoma (escc). in this study we aimed to study the  role of hpv in escc. patients and methods:in this study, 140 cases of escc were analyzed for the hpv dna by  polymerase chain reaction (pcr) using gp5+/gp6+ primers for l1 open reading frame (orf) to  am...