The avian embryo has been a classical model to study early development because the embryo is easily accessible for manipulation of embryonic cells and structures. The usefulness of the chicken embryo increased with the development of quail-chick transplantation techniques (1,2). The ability to transplant Japanese quail cells into a chicken embryo provides a method to trace the developmental fat...

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To identify the protein-tyrosine kinases that are expressed during chicken embryonic development, a 10-day chicken embryo cDNA expression library was screened with anti-phosphotyrosine antibodies. Of the positive clones isolated, many encoded the same protein-tyrosine kinase, which we designate Cek1 (chicken embryo kinase 1). Its amino acid sequence suggests that the Cek1 protein is a transmemb...

The fusion of myoblasts into multinucleated myotubes is a crucial step of muscle growth during development and of muscle repair in the adult. While multiple genes were shown to play a role in this process, a vertebrate model where novel candidates can be tested and analyzed at high throughput and relative ease has been lacking. Here, we show that the early chicken embryo is a fast and robust mo...

An apparent DNA-binding protein is described that is specific for cells transformed by avian acute leukemia viruses. This protein cannot be demonstrated in cells transformed by any of the avian sarcoma viruses or in cells infected with nontransforming avian retroviruses. The protein also is not detectable in noninfected quail or chicken embryo primary cultures or in noninfected chicken hematopo...

A lambda gt11 cDNA library from 10-day-old chicken embryo was screened immunologically using an antiserum against drebrins E1, E2 and A, proteins previously designated S5, S6 and S54, respectively. A cDNA clone for a common domain of drebrin was isolated. Northern blot analysis of chicken brain indicated that drebrin mRNAs are about 2.7 kilobases in molecular size and that expression of these p...

The effects in the chicken embryo assay of four Alternaria metabolites (alternariol [AOH], alternariol methyl ether [AME], altenuene [ALT], and tenuazonic acid [TA]) were investigated. Administered to 7-day-old chicken embryos by yolk sac injection, AOH, AME, and ALT caused no mortality or teratogenic effect at doses up to 1,000, 500, and 1,000 micrograms per egg, respectively. TA exhibited a c...

Protein kinase capable of phosphorylating 40S ribosomal protein S6 on serine residues has been detected in chicken embryo fibroblasts. This activity appears to be regulated in direct response to expression of pp60v-src in chicken embryo fibroblasts infected with a temperature-sensitive transformation mutant of Rous sarcoma virus. Partially purified S6 kinase was highly specific for S6 in 40S ri...

Radioadapted chicken embryo cells (X-irradiation in ovo with 10 cGy at the 14th day of development with priming periods of 24 h) were treated in vitro by challenge doses of 14 different DNA- and/or chromatin-interactive agents, including X-rays. A decrease in the cellular damage, as measured by scheduled DNA synthesis, was only observed with X-irradiation. Sedimentation of nucleoids as well as ...