High throughput (HT) platforms serve as a cost-efficient and rapid screening method for evaluating the effect of cell-culture conditions and screening of chemicals. We report the development of a HT cell-based microarray platform to assess the effect of culture conditions on Chinese hamster ovary (CHO) cells. Specifically, growth, transgene expression and metabolism of a GS/methionine sulphoxim...

Background A large number of recent publications demonstrate the effect of cell culture media on post-translational modifications of recombinant proteins [1]. In this study, aiming to extend the toolbox of media design beyond the commonly known media components, a large variety of cell culture compatible chemical components such as sugars in industrial relevant Chinese hamster ovary cell lines ...

Background Seven out of the ten top-selling biopharmaceuticals in 2011 are produced in Chinese Hamster Ovary (CHO) cells [1]. This tremendous commercial interest makes the development and application of strategies for cell line optimization, like gene overexpression or knockdown to enhance cell specific productivity and cellular growth, highly interesting. In this work, we investigated the knoc...

The understanding of the nutritional requirements for Chinese hamster ovary (CHO) cells and other immortalized cell lines was an early milestone in developing cell culture media. A key aspect of these early studies was defining those amino acids termed ‘essential’ for survival and growth of different cell lines. However, the amino acids essential for growth of cells in culture differ from those...

The widespread use of nanomaterials in vaccines, therapeutics, and industrial applications creates an increasing demand for understanding their ingestion by living cells. Researchers the field have called a more robust physical/chemical particle–cell interactions means to determine particles ingested per cell. Using superparamagnetic nanobeads, we measured beads cell quantified kinetics recepto...

Two types of serological assays are commonly used for the assessment of pertussis vaccine-induced antibodies; the Chinese hamster ovary cell (CHO cell) assay and the immunoglobulin G (IgG) anti pertussis toxin (PT) enzyme-linked immunosorbent assay (IgG anti-PT ELISA). Recently, both the techniques have been modified to improve performance with sera with interfering activity (CHO cell assay) or...

Background Follicle stimulating hormone (FSH) produced in recombinant Chinese hamster ovary (CHO) cells used for infertility treatment. Fetal Bovine Serum (FBS) in known as a factor in medium culture that provides the tolerance towards physiochemical variations and protects cells against apoptosis; on the other hand, FBS is the most expensive component in medium culture and collection that caus...