A total of 189 Acinetobacter baumannii isolates were collected in 2011 from a teaching hospital in Chongqing, China. Susceptibility data showed strains carrying integrons were significantly more resistant to all tested antibiotics that strains lacking integrons. Five types of gene cassettes belonging to class I integrons were identified in this study, and for the first time two types of gene ca...

OBJECTIVES Integrons are thought to play an important role in the spread of antibiotic resistance. This study investigates class 1 and 2 integron-positive methicillin-resistant coagulase-negative staphylococci strains isolated in Iran and characterizes their patterns of antimicrobial resistance. METHODS Hundred clinical isolates of coagulase-negative staphylococci were characterized for integ...

Gram-negative Pseudomonas spp. particularly P. aeruginosa is an important pathogen in hospitalized patients, contributing to the morbidity and mortality due its multiple resistance mechanisms. The present study was undertaken determine characteristics of isolated from pus patients admitted a local diagnostic center, Dhaka, Bangladesh. A total 14 clinical isolates were obtained. Detailed biochem...

OBJECTIVES To examine 397 strains of Salmonella enterica of human and animal origin comprising 35 serotypes for the presence of aadB, aphAI-IAB, aadA1, aadA2, bla(Carb(2)) or pse1, bla(Tem), cat1, cat2, dhfr1, floR, strA, sul1, sul2, tetA(A), tetA(B) and tetA(G) genes, the presence of class 1 integrons and the relationship of resistance genes to integrons and antibiotic resistance. RESULTS So...

OBJECTIVES Integrons are bacterial genetic elements that can capture and express genes contained in mobile cassettes. Integrons have been described worldwide in Gram-negative bacteria and are a marker of antibiotic resistance. We developed a specific and sensitive Taqman probe-based real-time PCR method with three different primer-probe pairs for simultaneous detection of the three main classes...

The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present study has revealed the sequence of a new gene, aadA5, integrated as a gene cassette together with the trimethoprim resistance gene dfr7 in a class 1 integron. The integron was located on a plasmid and was identified ...

Until the discovery of the Vibrio cholerae repeat (VCR), the gene capture and expression systems termed integrons had been typically associated with antibiotic-resistance gene cassettes with usually less than five genes in an array. A method is described for the cloning of the ends of large cassette arrays. Conserved restriction sites within VCRs facilitated the mapping by Southern hybridizatio...

Abstract Background Group II introns are mobile genetic elements used as efficient gene targeting tools. They function both ribozymes and retroelements. IIC the only class reported so far to be associated with integrons. In order identify group linked integrons CALINS (cluster of att C sites lacking a neighboring integron integrase) within halophiles, we mined for in 28 assembled metagenomes fr...

Conjugative transposons in Gram-negative bacteria have a significant role the dissemination of antibiotic-resistance-conferring genes between bacteria. This study aims to genomically characterize plasmids and conjugative carrying integrons clinical isolates Klebsiella pneumoniae. The genetic composition phenotypic assessment 50 multidrug-resistant K. pneumoniae from tertiary-care hospital (SQUH...