The cochaperone Sti1/Hop physically links Hsp70 and Hsp90. The protein exhibits one binding site for Hsp90 (TPR2A) and two binding sites for Hsp70 (TPR1 and TPR2B). How these sites are used remained enigmatic. Here we show that Sti1 is a dynamic, elongated protein that consists of a flexible N-terminal module, a long linker and a rigid C-terminal module. Binding of Hsp90 and Hsp70 regulates the...

In this paper we describe the design and implementation of a system called Web Interaction Display and Monitoring (WIDAM). We have developed a web based client-server application that offers several services: (i) real time monitoring of the user interaction to be used in synchronous playback (Synchronous Monitoring Service) (ii) real time observation by other users (Synchronous Playback Service...

To assist a solution to the problem of the test environment spanning multiple platforms, this paper proposes a decision support framework with the blackboard model to integrate all complementary features into a single automated test environment for multi-platform client/server applications. Before testing client/server applications, the input into this framework are testing tools with different...

How would you like to share files with another user without having to explicitly place them in a designated external location? The recent successes of (and controversies surrounding) Napster, Gnutella, and FreeNet have drawn attention to peer-to-peer computing, which allows precisely such interactions between information and service providers and their customers. Let’s take a brief look at peer...

The consistency issues have been well studied in term of methodologies and technologies for the most popular interaction paradigm, namely the client-server. In the agent’s world such a paradigm is used as much as another one, the notification-observation, in which an active system notifies its status changes to registered observer agents. In the client-server paradigm, when a client invokes a r...

HSP90 is a molecular chaperone that associates with numerous substrate proteins called clients. It plays many important roles in human biology and medicine, but determinants of client recognition by HSP90 have remained frustratingly elusive. We systematically and quantitatively surveyed most human kinases, transcription factors, and E3 ligases for interaction with HSP90 and its cochaperone CDC3...

When developing a (web) interface for a deductive database, functionality required by the client is provided by means of HTTP handlers that wrap the logical data access predicates. These handlers are responsible for converting between client and server data representations and typically include options for paginating results. Designing the web accessible API is difficult because it is hard to p...

Our research contributes to the quest for management action items that drive outsourcing management success. We hypothesize and empirically show that a certain piece of IT governance, the explicit documentation of roles and responsibilities of staff residing in the client firm’s retained organization contributes to social alignment in terms of interaction quality, shared knowledge, and trust be...