be a distinct class of accessory factors, which we term " plus-end-tracking proteins " or ϩTIPs. These MAPs are among the most conserved components of the MT cy-toskeleton. They localize to the MT plus end, and in living cells, they mark growing plus ends and thus give The Children's Hospital the appearance of " tracking. " Recently, studies from a Harvard Medical School variety of organisms ha...

PURPOSE The threshold estimation, learning effect, and between-algorithm differences of the Fast Swedish Interactive Thresholding Algorithm (SITA Fast), of the Humphrey Field Analyzer (HFA), and the Continuous Light Increment Perimetry (CLIP) strategy of the Oculus Twinfield perimeter were evaluated in damaged visual fields. METHODS Twenty-one glaucomatous patients with damaged visual fields ...

Cross-linking immunoprecipitation coupled with high-throughput sequencing (CLIP-Seq) has made it possible to identify the targeting sites of RNA-binding proteins in various cell culture systems and tissue types on a genome-wide scale. Here we present a novel model-based approach (MiClip) to identify high-confidence protein-RNA binding sites from CLIP-seq datasets. This approach assigns a probab...

The presentation of antigenic peptides by major histocompatibility complex (MHC) class II to CD4+ T cells is crucial to initiate immune responses. We developed a new system for delivery of an antigenic peptide to the MHC class II pathway, using the invariant chain (Ii). We designed a mutated human p33-form Ii, CLIP-substituted Ii, in which streptococcal M12p55-68 (RDLEQAYNELSGEA) was substitute...

Introduction. Laparoscopic and robot-assisted laparoscopic surgery are widely performed in urology field, so Hem-o-Lok clips are thus extensively used in the laparoscopic procedures. We describe the first case of Hem-o-Lok clip which migrated into the neobladder with calculus formation 26 months after laparoscopic orthotopic neobladder cystectomy, which causes symptoms of gross hematuria and fr...

PURPOSE To evaluate the efficacy and stability of the wrap-clipping methods as a reconstructive strategy in the treatment of unclippable cerebral aneurysms. MATERIALS AND METHODS Twenty four patients who had undergone wrap-clipping microsurgery were retrospectively reviewed. Type and morphology of the treated aneurysm, utilized technique for wrap-clip procedure, and clinical outcome with angi...

CLIP-seq experiments are currently the most important means for determining the binding sites of RNA binding proteins on a genome-wide level. The computational analysis can be divided into three steps. In the first pre-processing stage, raw reads have to be trimmed and mapped to the genome. This step has to be specifically adapted for each CLIP-seq protocol. The next step is peak calling, which...

Cytoplasmic linker protein (CLIP)-170 is a microtubule (MT) plus-end-tracking protein that regulates MT dynamics and links MT plus ends to different intracellular structures. We have shown previously that intramolecular association between the N and C termini results in autoinhibition of CLIP-170, thus altering its binding to MTs and the dynactin subunit p150(Glued) (J. Cell Biol. 2004: 166, 10...

This paper looks into a new direction in movie clips analysis –model based ranking of highlight level. A movie clip, containing a short story, is composed of several continuous shots, which is much simpler than the whole movie. As a result, clip based analysis provides a feasible way for movie analysis and interpretation. In this paper, clip-based ranking of highlight level is proposed, where t...