VASILEVA, V., E. VASILEV and R. TZONEV, 2016. Subterranean clover (Trifolium subterraneum L.) as a promising forage species in Bulgaria. Bulg. J. Agric. Sci., 22: 222–227 The paper presents an overview of the possibility to use the subterranean clover (Trifolium subterraneum L.) (an annual leguminous self-seeding species, widespread component in the pastures of the temperate areas of Central an...

White clover (Trifolium repens L.) is an allotetraploid species (2n = 4X = 32) that is widely distributed in temperate regions and cultivated as a forage legume. In this study, we developed expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers, constructed linkage maps, and performed comparative mapping with other legume species. A total of 7982 ESTs that could be assembled ...

Sheep were fed once daily with 300 or 600 g dried perennial ryegrass (Lolium perenne) or white clover (Trifolium repens). Total rumen contents were sampled once daily at various intervals up to 24 h after feeding for 6 d. Total weight of fresh rumen contents and dry matter (DM), organic matter and particulate matter concentrations were measured and the particle size distribution of rumen conten...

Improving drought tolerance through drought preconditioning is an important way to understand drought tolerance mechanism in plants. Some limited researches have focused on the gene expression patterns in conjunction with the underlying enzymes promoting drought tolerance and post-drought recovery in white clover. The objective of this study was to identify whether preconditioning-induced droug...

The input of Nitrogen (N) through symbiotic N2 fixation (SNF) in grass-clover mixtures was determined in an organic cropping system for grazing during 3 years. The mixture of perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.) was established by undersowing in spring barley (Hordeum vulgare L.) and maintained subsequently for two production years. Dinitrogen fixation w...

White clover is a short-lived perennial whose persistence is greatly affected by abiotic stresses, particularly drought. The aim of this work was to characterize its molecular response to water deficit and recovery following re-hydration to identify targets for the breeding of tolerant varieties. We created a white clover reference transcriptome of 16,193 contigs by deep sequencing (mean base c...

The experiment investigated the digestion of lipids from different forage silages in beef steers. Six Hereford x Friesian steers prepared with rumen and duodenal cannulas were given ad libitum access to a high-sugar grass silage, control grass silage, red clover silage, or mixtures of the red clover and each of the grass silages (50:50, DM basis). The experiment was conducted as an incomplete 5...

The endemic and rare Solanum vespertilio and S. lidii from the Canary Islands are notable in being andromonoecious, self compatible, highly heterandrous (with short >reward= anthers and one very long >pollination= anther), and bearing strongly zygomorphic corollas. Solanum vespertilio flowers are also tetramerous, and some display enantiostyly. Given their island distribution, uncommon for sola...

Twenty-four multiparous Holstein-Friesian dairy cows were used in a replicated 3×3 Latin square changeover design experiment to test the effects of changing from corn (Zea mays) silage to red clover (Trifolium pratense) silage in graded proportions on feed intakes, milk production, and whole-body N and P partitioning. Three dietary treatments with ad libitum access to 1 of 3 forage mixtures plu...