نتایج جستجو برای: cma3
تعداد نتایج: 209 فیلتر نتایج به سال:
چکیده زمینه و هدف: کمبود پروتامین و آسیب dna بر میزان بیان ژن ها در دوره ی جنینی اثر مهمی دارد. نگهداری اسپرم در محیط کشت موجب تغییرات مورفولوژیک هسته و آزاد شدن dna می گردد. با وجود این، ارتباط بین کمبود پروتامین و میزان آزاد سازی قطعات dna از اسپرم ها ی نگهداری شده در محیط کشت و حضور ژن dnmt1 در این قطعات مشخص نمی باشد. روش بررسی: آنالیز مایع سیمن طبق معیار who انجام شد. جهت تعیین کمبود پروتا...
Background The solid and colloidal particles in the range of 1-100 nm are called nanoparticles. Recently, one of the most important metal nanoparticles, with wide usage is gold nanoparticle. Based on the previous studies, gold nanoparticles have spermatotoxic effects and can destroy sperm motility. The aim of present study is the effect of gold nanoparticles on chromatin quality and sperm param...
background: evaluating the significance and the effects of plant-derived drugs on laboratory animal’s fertility was recognized. there was antioxidant activity reported from heracleum persicum (golpar).objective: current study aims to study the antioxidant effect of golpar extracts on sperm parameters and chromatin quality in mice.materials and methods: eighteen adult male mice were divided to 3...
Background: Selection of human spermatozoa prior to assisted reproduction is currently based on motility and morphology. Recently, Zeta method has been introduced for normal sperm selection based on sperm surface charges. In addition, one of the features of apoptosis is the externalization of phosphatidylserine (PS), which are normally present on the inner leaflet of the sperm plasma membrane. ...
This is the first report implementing combined density gradient centrifugation/Zeta (DGC/Zeta) sperm selection procedure for a couple with eleven previous intra cytoplasmic sperm injection/ in vitro fertilization (ICSI/IVF) failure cycles. Semen analysis was carried out according to World Health Organization criteria. Protamine deficiency, DNA fragmentation and morphology were assessed by chrom...
background: diabetes mellitus (dm), primary or idiopathic is a chronic disorder of the carbohydrate, lipid and protein metabolism. dm may impact male reproductive function at several levels. it is shown that dm has detrimental effects on sperm parameters in human and experimental animals. objective: the aim of this study was to observe the effects of diabetes on sperm parameters (viability, cou...
Tityus trivittatus Kraepelin, 1898 is the most medically important scorpion species of Argentina, and parthenogenetic populations are present in the major cities of this country. We performed a detailed cytogenetic analysis of specimens of three synanthropic parthenogenetic populations, all distant about 900 km from each other, using Ag-NOR, C-banding, DAPI/CMA3 staining and FISH with autologou...
In this paper we present, for the first time, a detailed karyotype characterization of a species of the genus Azteca (Dolichoderinae, Formicidae). Cerebral ganglia from Azteca trigona Emery, 1893 were excised and submitted to colchicine hypotonic solution and chromosomal preparations were analyzed through conventional staining with Giemsa, C-banding, silver nitrate staining (AgNO3) and sequenti...
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