The fluorescent antibody staining technique was adapted by i\Ioody, Goldman, and Thomason (J. Bacteriol., 72, 357, 1956) for the identification of Mllalleomyces pseudomallei and has been used to identify additional microorganisms. This note reports the application of the direct fluorescent staining technique to the detection and identification of coagulase-positive staphylococci. Cowan (J. Path...

Gravenkemper, Charles F. (University of Washington School of Medicine, Seattle), Jean L. Brodie, and William M. M. Kirby. Resistance of coagulase-positive staphylococci to methicillin and oxacillin. J. Bacteriol. 89:1005-1010. 1965.-Two strains resistant to methicillin were discovered among 541 strains of Staphylococcus aureus isolated in a clinical laboratory during a 1-yr period, and their pr...

The purpose of this study was to determine the minimum number of tests that could be used to differentiate between the coagulase-positive strains of staphylococcus: Staphylococcus aureus, Staphylococcus hyicus, and Staphylococcus intermedius. Eighty coagulase-positive strains of each of the three species were examined. The five tests conducted were growth on modified Baird-Parker agar, growth o...

Five hundred gram-positive clinical bacterial isolates were tested for susceptibility to mupirocin by broth microdilution and disk diffusion methods (5- and 10-micrograms disks). All but 1 of 330 staphylococci (including 100 oxacillin-resistant Staphylococcus aureus) were susceptible to less than or equal to 1.0 micrograms of mupirocin per ml. With a susceptible MIC breakpoint of less than or e...

Four-hundred milk samples from half udders of 20 flocks of native breed goats were asepticallycollected. The samples were examined by California mastitis test (CMT). Twenty-one (5.25%) of 400 wereCMT-positive. The CMT-positive samples were then cultured and the following bacteria were isolated:Staphylococcus aureus in 3 (14.28%), CNS (coagulase-negative staphylococci) in 14 (66.66%), streptococ...

To investigate the effects of fixation on the Gram reaction in the pituitary basiphil granules, frozen sections were made from fixed and unfixed glands, paraffin sections from frozen-dried glands, and paraffin sections in the usual way from whole glands, as well as from several pieces of glands embedded in one block. The fixatives were various well-known mixtures and single substances. Staphylo...

During inspection of 21 frozen breaded raw shrimp processors, 861 finished product units (retail packages) and 778 line samples were collected and analyzed bacteriologically. The bacteriological results for the finished product samples collected in plants with poor and good sanitary controls are presented and compared. The samples consisted of 4 to 38 units (retail packages) of the finished pro...

We have developed a PCR-based assay which allows the detection of staphylococci at the genus level by targeting the tuf gene, which encodes the elongation factor Tu. Degenerate PCR primers derived from consensus regions of several tuf genes were used to amplify a target region of 884 bp from 11 representative staphylococcal species. Subsequently, the entire nucleotide sequence of these amplicon...

•Single port access laparoscopy may be suggested for prophylactic salpingectomy in BRCA carriers.•Diode laser salpingectomy could preserve ovarian function as it can cut and coagulate with extreme precision and minimal thermal damage.