Fluorescence confocal laser scanning microscopy (CLSM) has revolutionized imaging of subcellular structures in biomedical research by enabling the acquisition of 3D time-series of fluorescently-tagged proteins in living cells, hence forming the basis for an automated quantification of their morphological and dynamic characteristics. Due to the inherently weak fluorescence, CLSM images exhibit a...

Confocal Scanning Laser Microscopy (CSLM) has advantages over conven tional light microscopy and electron microscopy. In particular the possibility to perform optical section ing, allowing the disturbance free observation of the three-dimensional internal structure, offers new possibilities in microstructural studies of food sys tems. The technique is further considered to be very valuable in t...

Confocal laser scanning microscopy (CLSM) was used in the reflection mode to characterize the surface texture (roughness) of sliced food surfaces. Sandpapers with grit size between 150 and 600 were used as height references to standardize the CLSM hardware settings. Sandpaper particle sizes were verified by scanning electron microscopy (SEM). The mean amplitude (in micrometers) of surface varia...

The measurement of micro components and microstructures leads to special requirements of measuring gauges. State-of-the-art Confocal Laser Scanning Microscopes (LSM) are suited to meet these requirements. The paper shows examples of effective and accurate analyses of micro tools, micro components, and micro structures with a LSM. It becomes clear, that measurements with a confocal LSM lead to v...

PURPOSE To visualize structural alterations of the cornea, iris, and lens in patients with exfoliation syndrome (XFS) using a noncontact in vivo laser scanning confocal microscope and to correlate these with the clinical features. METHODS The cornea, iris, and lens of 30 eyes with XFS were imaged using the Rostock Cornea Module of Heidelberg Retina Tomograph II (50x noncontact Nikon lens, an ...

Confocal scanning microscopy (CSM) is a much used and advantageous form of microscopy. Although CSM is superior to conventional microscopy in many respects, a major disadvantage is the complexity of the scanning process and the sometimes long time to perform the scan. In this thesis a novel non-scanning fluorescence confocal microscopy is investigated. The method uses a random timevarying speck...

We present a new particle image correlation technique for resolving nanoparticle flow velocity using confocal laser scanning microscopy (CLSM). The two primary issues that complicate nanoparticle scanning laser image correlation (SLIC)–based velocimetry are (1) the use of diffusion-dominated nanoparticles as flow tracers, which introduce a random decorrelating error into the velocity estimate, ...

Confocal laser scanning microscopy (CLSM) is being increasingly used for observing protein uptake in porous chromatography resins. Recent CLSM studies have revealed the possible existence of a nondiffusive protein transport mechanism. Observing protein uptake with CLSM requires labeling the protein with a fluorescent probe. This study examines the effect of the probe identity on the subsequent ...

Significant advances in fluorescence microscopy tend be a balance between two competing qualities wherein improvements in resolution and low light detection are typically accompanied by losses in acquisition rate and signal-to-noise, respectively. These trade-offs are becoming less of a barrier to biomedical research as recent advances in optoelectronic microscopy and developments in fluorophor...