Rationale: Our insights into physiological and pathophysiological cardiac excitation-contraction coupling has greatly benefited from significant advancement in optical technologies such as high-speed confocal microscopy. This has pushed pixel dwell times into the time domain of nanoseconds, resulting in low signal-to-noise ratios, which have limited data analysis and interpretation. Objective: ...

Zebrafish have long been utilized to study the cellular and molecular mechanisms of development by time-lapse imaging of the living transparent embryo. Here we describe a method to mount zebrafish embryos for long-term imaging and demonstrate how to automate the capture of time-lapse images using a confocal microscope. We also describe a method to create controlled, precise damage to individual...

Comprehensive microscopy of distal esophagus could greatly improve the screening and surveillance of esophageal diseases such as Barrett's esophagus by providing histomorphologic information over the entire region at risk. Spectrally encoded confocal microscopy (SECM) is a high-speed reflectance confocal microscopy technology that can be configured to image the entire distal esophagus by helica...

Confocal microscopy is routinely used for high-resolution fluorescence imaging of biological specimens. Most standard confocal systems scan a laser across a specimen and collect emitted light passing through a single pinhole to produce an optical section of the sample. Sequential scanning on a point-by-point basis limits the speed of image acquisition and even the fastest commercial instruments...

Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning digital techniques, in general—in addition to high sensitivity, fast data acquisition, great versatility 2D–4D image analyses—also opened technical possibilities combine with spectroscopic measurements. In this review, we focus attenti...

ct image quality has been affected by many factors such as kvp, ma, exposure time, alogorithm and section thickness. gantry tilt has the main role in visualization of the temporal bone anatomic structures. in this study, we tried to optimize these factors to determine the best gantry tilt. this study was performed on the human skull phantom and then extended to 15 patients. the phantom was made...

purpose : to assess and to compare stromal and endothelial changes at the cellular level in patients who had photorefractive keratectomy (prk) and laser in situ keratomileusis (lasik) using in vivo confocal microscopy methods : in this semiexperimental study, 32 eyes of 16 patients (4 males and 12 females) with low to moderate myopia [-1.00 to -4.50 diopters (d)] or low to moderate myopic astig...

The purpose of this study was to visualize penetrance self dissolving microneedles into different layers the epidermis, subsurface anatomical changes and calculate distance between deposition drugs in dermis. We recruited 8 subjects age groups better understand how microneedling patches containing hyaluronic acid botulinum polypeptide-1 work under eye reduce wrinkles. Clinical pictures were tak...

Fading is one of the major obstacles to reliable observation in fluorescence microscopy. Using a confocal laser scanning microscope (CLSM) coupled to a computer, we quantitatively measured fading of fluorescence to formulate an equation, evaluated the anti-fading ability of several anti-fading media, and restored the faded images to the original level according to this equation. NIH 3T3 cells w...