A 10 L continuous stirred tank reactor (CSTR) system was developed for a two-stage hydrogen fermentation process with an integrated alkaline treatment. The maximum hydrogen production rate reached 218.5 mL/L h at a cornstalk concentration of 30 g/L, and the total hydrogen yield and volumetric hydrogen production rate reached 58.0 mL/g-cornstalk and 0.55-0.57 L/L d, respectively. A 10 L up-flow ...

The effects of probiotics, yucca extract, monensin and L-cysteine on rumen methanogenesis were determined by in vitro incubation using mixed bovine rumen microorganisms with ground Bermuda grass hay as a substrate. Two forms of probiotics, yucca extract, monensin and L-cysteine were tested during 24 hour anaerobic incubations. Methane and carbon dioxide production and fermentation characteristi...

SUM]IARY The release of cytoplasmic enzymes from vital populations of ELD ascites tumor cells and cultivated strain L clone 9~9 cells has been studied under aerobic and anaerobic conditions in media containing glucose and serum albumin. The released enzyme activities were used as markers for the release of intracellular proteins. The catheptic, glutathione reductase, and lactic dehydrogenase ac...

The potential ecological impacts of anaerobic degradation of vegetable oil on freshwater sediments were investigated. Sediment toxicity was evaluated using two regulatory biotests: the Microtox Solid Phase Test and an amphipod (Hyalella azteca) bioassay. The results of the Microtox test showed that the toxicity of the vegetable-oil-contaminated sediments (about 17–33 g oil/kg dry sediments) inc...

1 2 A Central Composite Face design was used to study growth and tyramine production 3 by two strains of lactic acid bacteria, Lactobacillus brevis CECT 4669 and Enterococcus 4 faecium BIFI-58. The effects of five physicochemical factors (incubation temperature and 5 time, environmental pH, added tyrosine concentration, and pyridoxal-5-phosphate (PLP) 6 supplementation) on cell growth and tyram...

Consecutive batch culture, a technique which involves sequential transfer of cultures to fresh medium at regular intervals, was used to establish mixed ruminal-microbial populations in an anaerobic medium containing highly digestible hay. Once volatile fatty acid production was stable, perturbations were imposed in consecutive cultures by the addition of one of each of the following pentoses or...

Bacteria that mediate the anaerobic oxidation of ammonium (anammox) have been detected in natural ecosystems, as well as various wastewater treatment systems. In this study, sludge from a particular landfill leachate anaerobic treatment system was selected as the incubation seed for anammox microorganism enrichment owing to its possible anammox activity. Transmission electron microscopy observa...

Intertidal individuals of Mytilus galloprovincialis were exposed to anaerobiosis in laboratory at 22°C and a set of biochemical metabolites and survival potential determined. Differences in survival potential between individuals emersed or kept in oxygen-free seawater were residual according to ST50 values (survival time, P≈0.05) but emersed individuals survived significantly longer when consid...

When spinach (Spinacia oleracea) leaf discs were incubated in a dark anaerobic environment, the chlorophyll fluorescence yield was much increased relative to the aerobic control. Occasionally, the fluorescence yield of the darkened anaerobic samples approached 80% of the maximum fluorescence. The anaerobic incubation period also induced in a leaf disc the capacity to exhibit a low light-mediate...