Purpose: Neuroscience studies suggest that Chronic Low Back Pain (CLBP) is associated with central sensitization, and maladaptive reorganization of the brain; this introduced a new target for LBP treatment. Studies revealed that cortical and peripheral electrical stimulation can be beneficial in regulating brain neuronal activity. However, there is a scarcity of evidence to support the effects ...

The organization of actin microfilaments was studied by immunofluorescence isolated from sunflower hypocotyls and cultured in an agarose matrix. Remov wall completely disrupted the actin cytoskeleton, which became progressively into cortical microfilament arrays and actin cables during protoplast culture. protoplasts with Arg-Gly-Asp motif-containing peptides, to inhibit putative cell the agaro...

Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory-inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocyt...

introduction: standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. however, since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. this study, therefore, was conducted to assess whe...