ICP4 is an activator of herpes simplex virus early and late gene transcription during infection and in vitro can efficiently activate the transcription of a core promoter template containing only a TATA box and an initiator element. In this study, we noted that the extent of activation by ICP4 in vitro was highly dependent on the purity of TFIID when recombinant TFIIB, TFIIE, and TFIIF were use...

Buxus sempervirens L. (European Box, Buxaceae) has been used in ethnomedicine to treat malaria. In the course of our screening of plant extracts for antiprotozoal activity, a CH2Cl2 extract from leaves of B. sempervirens showed selective in vitro activity against Plasmodium falciparum (IC50 = 2.79 vs. 20.2 µg/mL for cytotoxicity against L6 rat cells). Separation of the extract by acid/base extr...

The protease inhibitive assay for heavy metals is a novel and robust detection method suitable for preliminary screening method for detecting heavy metals in agriculture and environment samples. Stem bromelain has been previously showed to be sensitive towards mercury and copper. However, commercial preparation is in crude form and contains possible interfering entities. Using ion exchange and ...

Seven crude oil samples recovered from wells of El Hamd and Bakr oil Fields locates in Egyptian central Gulf of Suez were subjected for Description, bulk composition of Maltene Fraction and hydrocarbons distribution. The Comparison of collected crude oils was achieved using Bulk parameters and Specific parameters, the first ones refer to properties of the whole sample such as API gravity, sulfu...

Euphorbia wallichii a perennial herb growing mainly in Himalayas has been widely used in folk medicines for its medicinal properties. In the present study, the crude methanolic root extract (CME) and its fractions; n-Hexane Fraction (NHF), n-Butanol Fraction (NBF), Chloroform Fraction (CHF), Ethyl acetate Fraction (EAF) and Aqueous Fraction (AQF) of this plant specie were investigated for antio...

Low-temperature oxidation (LTO) of four Arabian crudes as well as blends of naphtha with a super-light crude was investigated employing differential thermal analysis. The mass fraction of un-saturates in the reactants varied between 0.2 and 0.9. All reactants showed LTO peaks between 230 and 264 oC; heat flow at the peak, however, varied widely. The data revealed a clear increase in LTO-generat...

A new pyrrolidine alkaloid codonopsinol C (1), and pyrrolidine alkaloidal glycoside, codonopiloside A (2), were isolated from the roots of Codonopsis pilosula, along with four known pyrrolidine alkaloids, codonopsinol A (3), codonopsinol B (4), codonopyrrolidium B (5), and radicamine A (6). The structures of the new compounds were established by acid hydrolysis and spectroscopic methods. We des...

The concentration of alkaloidal constituents of Fumaria indica at different stages of its life span were estimated. The total alkaloid content was found maximum between 21 - 40 days whereas a mark able variations in the concentrations of pure individual alkaloids at different stages were observed.