Krüppel-like factor 8 (KLF8) has been strongly implicated in breast cancer metastasis. However, the underlying mechanisms remain largely unknown. Here we report a novel signaling from KLF8 to C-X-C cytokine receptor type 4 (CXCR4) in breast cancer. Overexpression of KLF8 in MCF-10A cells induced CXCR4 expression at both mRNA and protein levels, as determined by quantitative real-time PCR and im...

The expression of Cxcr4 mRNA that encodes the receptor for the chemokine Sdf1 was studied during mouse brain development using in situ hybridization, from E9.5 to maturity at P21. At embryonic stages, expression is prominent in ventricular zones of stem cell proliferation. This abates during the postnatal period in parallel to the depopulation of ventricular zones. In addition, the Cxcr4 gene i...

BACKGROUND Chemokine receptors have been shown to play an important role in the development and metastatic spread of various malignancies. In this study, the gene expression profile of some key chemokine receptors involved in metastasis has been investigated in esophageal and breast cancer cell lines. METHODS In a descriptive study, gene expression profile of CCR1, CCR6, CCR7, CCR9, CXCR1, an...

Mayorga M, Kiedrowski M, Shamhart P, Forudi F, Weber K, Chilian WM, Penn MS, Dong F. Early upregulation of myocardial CXCR4 expression is critical for dimethyloxalylglycine-induced cardiac improvement in acute myocardial infarction. Am J Physiol Heart Circ Physiol 310: H20 –H28, 2016. First published October 30, 2015; doi:10.1152/ajpheart.00449.2015.— The stromal cell-derived factor-1 (SDF-1):C...

It has been shown that p210 significantly impairs CXCR4 signaling. We report here that the migratory response to SDF-1 was profoundly altered in blast crisis, whereas chronic-phase CD34 cells migrated normally to this chemokine. This migratory defect was associated with a low CXCR4 membrane expression. In vitro STI-571 treatment of CD34 cells from patients in blast crisis markedly increased the...

SDF-1 and its primary receptor, CXCR4, are highly expressed in the embryonic central nervous system (CNS) and play a crucial role in brain architecture. Loss of SDF-1/CXCR4 signaling causes abnormal development of neural stem/progenitor cells (NSCs/NPCs) in the cerebellum, hippocampus, and cortex. However, the mechanism of SDF-1/CXCR4 axis in NSCs/NPCs regulation remains unknown. In this study,...

The purpose of this study was to investigate the regulatory effects of breast cancer fibroblasts (BCFs) vs. normal mammary fibroblasts (NMFs) on mammosphere formation and stem cell-related gene expression in breast cancer cells. Breast cancer cells (MCF-7) were cultured in suspension to generate primary and secondary mammospheres. The proportion of CD44+/CD24low/- cells was assessed by flow cyt...

PURPOSE CXCR4 receptor and its unique ligand, the CXCL12 chemokine, have been recently implicated in cancer metastasis. Evidence about the role of CXCR4/CXCL12 axis has been reported in several cancers including melanoma. Our goal was to investigate if CXCR4 expression has a prognostic value in malignant melanoma. EXPERIMENTAL DESIGN Immunohistochemical expression of CXCR4 was evaluated on 71...

The aim of the present study was to investigate the expression of the chemokine receptor CXCR4 in human bladder carcinoma cells and its possible role in their metastatic growth in vitro. Immunofluorescent imaging and flow cytometry were performed to examine the expression of CXCR4 in the EJ and EJ-M3 cell lines. A highly specific CXCR4 antagonist, AMD3100, was used to inhibit metastatic growth,...

CXCR4 chemokine receptors retain hematopoietic progenitors and leukemia cells within the marrow microenvironment. We prospectively evaluated the prognostic implication of CXCR4 in 90 consecutive patients with acute myelogenous leukemia (AML) by flow cytometry. Patients were divided into groups with low (n=32), intermediate (n=26), or high (n=32) CXCR4 expression, as defined by CXCR4 mean fluore...