ABSTRACT Among a variety of hybrids produced in Brazil, tambatinga is obtained from the crossing tambaqui female with pirapitinga male. Although rapid weight gain less time an attractive characteristic commercial viewpoint, information on its nutritional requirements, especially amino acid limited. As corn and soybean meal-based diets available for fish contain deficient levels essential methio...

Our objectives were to study how cysteamine, cystine, and cumulus cells (CCs), as well as oocytes interact to increase oocyte intracellular glutathione (GSH) and thereby to establish an efficient in vitro maturation system for cumulus-denuded oocytes (DOs). Using M16 that contained no thiol as maturation medium, we showed that when supplemented alone, neither cystine nor cysteamine promoted GSH...

PURPOSE To develop an imaging technique that maps the distribution of free cystine in the rat lens at subcellular resolution and enables cystine accumulation to be compared with the expression of the cystine-glutamate exchanger (Xc-). METHODS Whole lenses were fixed, cryoprotected, and then cryosectioned in either an equatorial or axial orientation. Sections were double labeled with either a ...

Cystine and cysteine are transported by energy-dependent, mediated processes in human gut. When either of these amino acids is transported, only cysteine is recovered intracellularly, indicating that cystine is reduced to cysteine after achieving an intracellular location. In contrast to results with cystine, cysteine uptake is not defective in gut from cystinuric patients, nor do lysine and ar...

Escherichin coli strain W is capable of actively transporting L-cystine as well as the structurally related compound OL,Ediaminopimelic acid. Cystine transport is mediated by two systems distinguishable on the basis of specificity and affinity for substrate. The cystine general system (K, = 3 x 10e7 M) also transports diaminopimelic acid and is inhibited by a variety of analogues, while the cys...

The earliest indication for the need of an organic sulfur complex in the diet came in 1915 from the work of Osborne and Mendel (I), who showed that when the proportion of casein in the diet of the rat was reduced from 18 to 9 per cent the resulting impairment of growth could be corrected by a supplement of cystine equal to 3 per cent of the casein. This finding was confirmed in many laboratorie...

We identify three dimeric proteins whose mechanostability is anisotropic and should exceed 1 nN along some directions. They come with distinct mechanical clamps: either shear-based, or involving a cystine slipknot, or due to dragging of a cystine plug through a cystine ring. The latter two mechanisms are topological in nature; the cystine plug mechanism has not yet been discussed but it turns o...

The transport of cystine has been investigated in mouse peritoneal macrophages cultured in vitro. The transport activity for cystine was very low in freshly isolated macrophages but was potently induced during culture in the presence of bacterial lipopolysaccharide (LPS) at concentrations as low as 0.1 ng/ml. The transport activity for cystine was enhanced when the cells were incubated with tum...

Gorini, Luigi (Harvard Medical School, Boston, Mass.). Effect of l-cystine on initiation of anaerobic growth of Escherichia coli and Aerobacter aerogenes. J. Bacteriol. 82:305-312. 1961.-Under anaerobic conditions Escherichia coli and Aerobacter aerogenes, inoculated in a mineral-citrate-glucose medium at densities up to 10(6) bacteria per ml, exhibit a long lag, or fail to initiate growth at a...

Uptake of cystine was investigated in mouse peritoneal macrophages. The rates of the uptake of cystine in resident macrophages or macrophages elicited by some irritants were very low, but a drastic increase was observed when the cells were cultured in vitro. This increase was time-dependent and required protein synthesis. In macrophages elicited by thioglycollate broth, the rate of the uptake o...