purification and isolation of cellular target proteins for monoclonal antibody (mab) production is a difficult and time-consuming process. immunization of mice with murine cell lines stably transfected with genes coding for xenogenic target molecules is an alternative method for mouse immunization and mab production. here we present data on transfection efficiency of some commercial reagents us...

Cytometry Part AVolume 99, Issue 4 Volume 99A, Number 4, April 2021 Cover ImageFree Access Image First published: 01 https://doi.org/10.1002/cyto.a.24075AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text full-text accessPlease review our Terms and Conditions of Use check box below share version article.I have read accept the Wiley...

Background: Invariant natural killer cells (iNKT) are an important immunoregulatory T cell subset. Currently several flow cytometry-based approaches exist for the identifi-cation of iNKT cells, which rely on using the 6B11 monoclonal antibody or a combina-tion of anti-Vα24 and anti-Vβ11 antibodies. Objective: The aim of this study was to compare the ability of two flow cytometry-based methods f...

The objective of this study was to evaluate the performance a small footprint benchtop somatic cell counter based on image cytometry (LactiCyte HD; Page and Pedersen International Ltd., Hopkinton, MA) against flow cytometer employed at regional dairy herd improvement (DHI) laboratory. Milk samples collected during monthly DHI testing were split into 2 samples. One sample evaluated using (Bentle...