In eukaryotic cells the final maturation of ribosomes occurs in the cytoplasm, where trans-acting factors are removed and critical ribosomal proteins are added for functionality. Here, we have carried out a comprehensive analysis of cytoplasmic maturation, ordering the known steps into a coherent pathway. Maturation is initiated by the ATPase Drg1. Downstream, assembly of the ribosome stalk is ...

The first meiotic cell division (meiotic maturation) of the dictyate stage of mouse oocytes, removed from the follicle, resumes spontaneously in vitro. This study indicates that estramustine phosphate reversibly blocks meiotic maturation by inhibiting germinal vesicle breakdown. Oocyte cryostat sections were stained with anti-tubulin and two antibodies (anti-MAP1: JA2 and 5051) which decorated ...

Abstract Cell division is a central and essential process in most bacteria, also due to its complexity highly coordinated nature, it has emerged as promising new antibiotic target pathway recent years. We have previously shown that ADEP antibiotics preferably induce the degradation of major cell protein FtsZ, thereby primarily leading depletion cytoplasmic FtsZ pool needed for treadmilling ring...

The oocyte maturation includes nuclear maturation and cytoplasmic maturation before fertilization. Moreover, the oocyte nucleus, the cytoplasm and other cell organelles occur changes, zona pellucida (ZP) synchronously undergoes biochemical and structural modifications in the final maturation phase of oocyte prior to fertilization (ZP maturation). It is reported that ZP modifications, such as gl...

Some aspects of oxidative metabolism during cytoplasmic maturation of the Bufo arenarum oocyte have been studied. During the autumn-winter period (immature oocyte), carbohydrates are degraded through the glycolytic pathway, followed by the classical tricarboxylic acid cycle. During the spring--summer period (mature oocyte), carbohydrates are mainly used through the pentose phosphate cycle, whil...