نتایج جستجو برای: cytoskeleton dynamics

تعداد نتایج: 462913  

2015
Kevin J. Hamill Sho Hiroyasu Zachary T. Colburn Rosa V. Ventrella Susan B. Hopkinson Omar Skalli Jonathan C. R. Jones

The migration of keratinocytes in wound healing requires coordinated activities of the motility machinery of a cell, the cytoskeleton, and matrix adhesions. In this study, we assessed the role of alpha actinin-1 (ACTN1), one of the two alpha actinin isoforms expressed in keratinocytes, in skin cell migration via a small hairpin RNA-mediated knockdown approach. Keratinocytes deficient in ACTN1 e...

Journal: :Frontiers in immunology 2016
William A. Comrie Janis K. Burkhardt

It is well known that F-actin dynamics drive the micron-scale cell shape changes required for migration and immunological synapse (IS) formation. In addition, recent evidence points to a more intimate role for the actin cytoskeleton in promoting T cell activation. Mechanotransduction, the conversion of mechanical input into intracellular biochemical changes, is thought to play a critical role i...

2016
Ghada Alhussein Aya Shanti Ilyas A. H. Farhat Sara B. H. Timraz Noaf S. A. Alwahab Yanthe E. Pearson Matthew N. Martin Nicolas Christoforou Jeremy C. M. Teo

The significant gap between quantitative and qualitative understanding of cytoskeletal function is a pressing problem; microscopy and labeling techniques have improved qualitative investigations of localized cytoskeleton behavior, whereas quantitative analyses of whole cell cytoskeleton networks remain challenging. Here we present a method that accurately quantifies cytoskeleton dynamics. Our a...

Journal: :Biophysical journal 2015
Cornelius Kronlage Marco Schäfer-Herte Daniel Böning Hans Oberleithner Johannes Fels

Contact-mode atomic force microscopy (AFM) has been shown to reveal cortical actin structures. Using live endothelial cells, we visualized cortical actin dynamics simultaneously by AFM and confocal fluorescence microscopy. We present a method that quantifies dynamic changes in the mechanical ultrastructure of the cortical actin web. We argue that the commonly used, so-called error signal imagin...

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