Endosomal escape is a rate limiting step for many promising therapeutics, including cyclic dinucleotide (CDN) agonists of the stimulator interferon genes (STING) pathway. In article number 2001056, John T. Wilson and co-workers synthesize screen novel library polymers with pH-responsive, membrane-destabilizing activity evaluate their capacity to enhance cytosolic delivery CDNs, resulting in ide...

A fresh water protozoan Paramecium multimicronucleatum adapted to a given solution was found to swell until the osmotic pressure difference between the cytosol and the solution balanced the cytosolic pressure. The cytosolic pressure was generated as the cell swelled osmotically. When either one or both of these pressures was somehow modified, cell volume would change until a new balance between...

The interior of a cell is very crowded (up to 35% protein by weight), with significant consequences for diffusive transport. The possible trajectories of a given tracer particle are constrained by the labyrinth of obstacles. For a static obstacle configuration, a percolation transition takes place at a certain critical obstacle density, so that above this density no long-range transport is poss...