We describe a recently developed in vitro assay for the assessment of the cytotoxicity of gaseous materials. The method is based on the pollen tube growth (PTG) test suited to determine the cytotoxicity of non-gaseous, water-soluble substances (Kristen and Kappler, 1995). Instead of the conventional PTG test, we used a semi-automatic, computer-controlled multi-channel device for the injection o...

Objective: To evaluate the cytotoxicity and antibacterial effect of bleached turmeric hydro-alcoholic extractcompared to sodium hypochlorite as an endodontic irrigant. Material Methods: Cytotoxicity was evaluatedon human skin fibroblasts using MTT assay. The irrigants were tested at 1, 5- 15-minutes time intervals. Aftercontact time, solution added well plates incubated. After incubation period...

INTRODUCTION This study aimed to compare the cytotoxicity of MTA Fillapex, AH-26 and Apatite root canal sealers at different times after mixing. METHODS AND MATERIALS In this in vitro study, MTA Fillapex, AH-26 and Apatite root canal sealer were spilled uniformly by 40 µm mesh in a 96-well plate. Then, human fetal foreskin fibroblast cell line (HFFF2) were added to each sealer cell culture me...

This paper describes the in vitro cytotoxicity assessment of single walled carbon nanotubes (SWCNT) on A549 cells, a human lung cell line. Cellular viability was determined using the alamar blue (AB), neutral red (NR) and MTT assays, which evaluated metabolic, lysosomal and mitochondrial activity respectively. In addition, the total protein content of the cells was measured using the coomassie ...

The cytotoxicity of the biomaterials is a key issue that should be addressed prior to pre-clinical applications. This study was designed to evaluate and compare the cytotoxixity of two forms of bioactive glasses:nanopowder and micropowder. Human HGF1-PI53 gingival fibroblast cells were used to evaluate the cytotoxicity of 0.5, 1, 1.5, 2, 5, 10, 15 and 20 mg/ mL concentrations of the two bioacti...

BACKGROUND The objective of this study was to investigate the toxicity of Lantana camara methanol extract. METHODS In order to evaluate the toxicity of Lantana camara, the acute toxicity of the methanolic extract on adult mice and cytotoxicity test on Vero cell line were investigated. A fixed large dose of 2 g/kg body weight of L. camara leaf extract was administrated by a single oral gavage ...

Many medicinal plants have been reported to exhibit protective effects against many physiological diseases as a result of their phytochemical components which are effective antioxidants. This study was aimed at comparing the phenolic and flavonoids contents, antioxidant cytotoxic activities methanol extract Jatropha curcas seeds leaves. The cytotoxicity level J. assessed using brine shrimp leth...

BACKGROUND Probiotic microorganisms are receiving increasing interest for use in the prevention, treatment, or dietary management of certain diseases, including antibiotic-associated diarrhea (AAD). Clostridium difficile is the most common cause of AAD and the resulting C. difficile - mediated infection (CDI), is potentially deadly. C. difficile associated diarrhea (CDAD) is manifested by sever...

background: angiogenesis, the formation of new blood vessels from preexisting ones, is one of the most important physiological and pathological processes occur in the body. in the pathological conditions such as tumor growth, psoriasis, corneal neovascularization and rheumatoid arthritis, angiogenesis is crucially required for the devolopment of the disease. zataria multiflora as a member of la...