The carbohydrate-binding properties of the Datura stramonium seed lectin were studied by equilibrium dialysis, quantitative precipitation of natural and synthetic glycoproteins, and hapten inhibition of precipitation. The dimeric lectin (Mr = 86,000) possesses two carbohydrate-binding sites for N,N'N",N"'- tetraacetylchitotetritol /mol protein, with an apparent Ka = 8.7 X 10(3) M-1 at 4 degrees...

The present study aimed at determining selected alkaloid components in the nectar of Datura species, to elucidate whether the alkaloid content of the floral nectar can lead to intoxication. A simple and rapid liquid chromatography coupled with electrospray mass spectrometry analysis was developed for the quantitative determination of atropine and scopolamine, the main toxic alkaloids of the Dat...

Aim: Evaluation of effect shodhana process datura seed (Datura stramonium) antioxidant activity. Methods: The was purified with two different processes viz. cow’s urine, and milk by Dolayantra method. Antioxidant activity evaluated in-vitro 1,1-Diphenyl-2-picryl hydrazyl (DPPH) radicals scavenging Ferric reducing power ability (FRPA) assay. Result: cow urine (CUD) showed a significant in inhibi...

Objective: To analyze the clinical features and prognosis of datura poisoning in children.

The aqueous extracts of twenty plants were screened by agar diffusion methods for their antibacterial activity against Rathyibacter tritici, a causal organism of tundu diseases of wheat. The combined extracts of leaf extracts of Datura stramonium and stem extracts of Acacia catechu in general showed a strong enhancement in activities over the individual extracts of leaves extracts of Datura str...

Zahra SHAKERAN, Mehrnaz KEYHANFAR*, Gholamreza ASGHARI, Mustafa GHANADIAN Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical ...

The complete amino acid sequences of [2Fe-2S] ferredoxins from Scopolia japonica and Lycium chinense have been determined by automated Edman degradation of the entire Cm-proteins and of the peptides obtained by enzymatic digestions. These two ferredoxins exhibited only 2-7 differences in the amino acid sequence when compared to the Datura-ferredoxins (D. stramonium, D. metel, and D. arborea), a...

The antimycotic potential of Datura metel (syn. Datura alba Nees.) was investigated in vitro against Ascochyta rabiei, the cause of chickpea blight disease. The pathogen was exposed to various n-hexane concentrations (1, 2, 3 and 4% w/v) of shoot and root extracts of D. metel using poisoning food technique. All the employed concentrations of both root and shoot extracts significantly suppressed...